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Literature DB >> 1191260

Evidence against the proposed interaction of thionitrobenzoate with protein disulphide bonds.

Abstract

Thionitrobenzoate was prepared from 5,5'-dithiobis-(2-nitrobenzoic acid), and its reaction with several disulphide-containing proteins was examined under various conditions. No evidence for any cleavage of disulphide bonds was obtained. The claim by Robyt et al. [Arch. Biochem. Biophys. (1971) 147, 262-269] that thionitrobenzoate allows the quantitative determination of disulphide bonds in proteins is not substantiated.

Entities: Chemical

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Year: 1975 PMID： 1191260 PMCID： PMC1165614 DOI： 10.1042/bj1490281

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

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References

13 in total

1. A critical reexamination of the reaction of sulfite with DTNB.

Authors: P D Weitzman
Journal: Anal Biochem Date: 1975-04 Impact factor: 3.365

2. The reactions of inter- and intra-chain disulphide bonds in proteins with sulphite.

Authors: R CECIL; R G WAKE
Journal: Biochem J Date: 1962-03 Impact factor: 3.857

3. Preparation of dihydrolipoamide by electrolytic reduction.

Authors: P D Weitzman; J K Hewson; M G Parker
Journal: FEBS Lett Date: 1974-07-01 Impact factor: 4.124

4. Sulfhydryl and disulfide groups of platelet membranes. II. Determination of disulfide groups.

Authors: Y Ando; M Steiner
Journal: Biochim Biophys Acta Date: 1973-06-07

5. Letter: A note on the molar absorptivity of reduced Ellman's reagent, 3-carboxylato-4-nitrothiophenolate.

Authors: H B Collier
Journal: Anal Biochem Date: 1973-11 Impact factor: 3.365

6. Secondary SH-disulfide exchange reactions following the modification of nucleophilic SH-groups in D-glyceraldehyde-3-phosphate dehydrogenase with DTNB.

Authors: L Boross
Journal: Acta Biochim Biophys Acad Sci Hung Date: 1969

Evidence against the proposed interaction of thionitrobenzoate with protein disulphide bonds.

1. A critical reexamination of the reaction of sulfite with DTNB.

2. The reactions of inter- and intra-chain disulphide bonds in proteins with sulphite.

3. Preparation of dihydrolipoamide by electrolytic reduction.

4. Sulfhydryl and disulfide groups of platelet membranes. II. Determination of disulfide groups.

5. Letter: A note on the molar absorptivity of reduced Ellman's reagent, 3-carboxylato-4-nitrothiophenolate.

6. Secondary SH-disulfide exchange reactions following the modification of nucleophilic SH-groups in D-glyceraldehyde-3-phosphate dehydrogenase with DTNB.

7. The electroreduction of the disulphide bonds of insulin and other proteins.

8. Study of correlation between structural motility and reactivity of SH groups in alpha-amylase.

9. Thermal motion as the rate-limiting step in the superprecipitation of actomyosin gels.

10. The reaction of papain with Ellman's reagent (5,5'-dithiobis- (2-nitrobenzoate) dianion).