| Literature DB >> 1191252 |
Abstract
The chemical nature of the important new chromogen ABTS [2,2'-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid)] is described together with an account of the redox and spectroscopic properties of the system ABTS--H2O2--peroxidase. Keq. is calculated and a study of the steady-state kinetics over a whole range of substrate concentrations is reported. By using novel methods of kinetic analysis, an interpretation of the results is given which requires some extension of the classical peroxidase mechanism.Entities:
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Year: 1975 PMID: 1191252 PMCID: PMC1165190 DOI: 10.1042/bj1450093
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857