Literature DB >> 11900643

Somatic cell cloning without micromanipulators.

G Vajta1, I M Lewis, P Hyttel, G A Thouas, A O Trounson.   

Abstract

Until now, micromanipulators have been regarded as indispensable for somatic cell nuclear transfer. This paper describes an improved zona-free nuclear transfer procedure with manual bisection of oocytes, selection of cytoplasts by Hoechst staining, and two-step fusion of somatic cells from primary granulosa cell cultures with two cytoplasts. Blastocyst rates in the three systems tested for zona-free embryo culture were 0%, 18%, and 36% for microdrops, well of the wells (WOW system), and microcapillaries (GO system), respectively. This simple, rapid, and inexpensive procedure may become a useful alternative to the existing techniques for somatic cell nuclear transfer for large-scale application of the technology.

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Year:  2001        PMID： 11900643     DOI： 10.1089/15204550152475590

Source DB:  PubMed          Journal:  Cloning        ISSN： 1520-4553


  21 in total

1.  Simple, fast, and efficient method of manual oocyte enucleation using a pulled Pasteur pipette.

Authors:  S M Hosseini; F Moulavi; V Asgari; A Shirazi; A H Abazari-Kia; H R Ghanaei; M H Nasr-Esfahani
Journal:  In Vitro Cell Dev Biol Anim       Date:  2013-07-04       Impact factor: 2.416

Review 2.  Factors and molecules that could impact cell differentiation in the embryo generated by nuclear transfer.

Authors:  Renata Simões; Arnaldo Rodrigues Santos
Journal:  Organogenesis       Date:  2017-10-02       Impact factor: 2.500

3.  Handmade cloned transgenic piglets expressing the nematode fat-1 gene.

Authors:  Peng Zhang; Yidi Zhang; Hongwei Dou; Jingdong Yin; Yu Chen; Xinzhi Pang; Gabor Vajta; Lars Bolund; Yutao Du; Runlin Z Ma
Journal:  Cell Reprogram       Date:  2012-06       Impact factor: 1.987

4.  Development, Characterization, and Pluripotency Analysis of Buffalo (Bubalus bubalis) Embryonic Stem Cell Lines Derived from In Vitro-Fertilized, Hand-Guided Cloned, and Parthenogenetic Embryos.

Authors:  Syed Mohmad Shah; Neha Saini; Syma Ashraf; Mohammad Zandi; Radhey Sham Manik; Suresh Kumar Singla; Prabhat Palta; Manmohan Singh Chauhan
Journal:  Cell Reprogram       Date:  2015-07-13       Impact factor: 1.987

5.  High developmental potential in vitro and in vivo of cattle embryos cloned without micromanipulators.

Authors:  Lleretny Rodríguez; Felipe I Navarrete; Heribelt Tovar; José F Cox; Fidel Ovidio Castro
Journal:  J Assist Reprod Genet       Date:  2008-01-18       Impact factor: 3.412

6.  Hemizygous minipigs produced by random gene insertion and handmade cloning express the Alzheimer's disease-causing dominant mutation APPsw.

Authors:  Peter M Kragh; Anders Lade Nielsen; Juan Li; Yutao Du; Lin Lin; Mette Schmidt; Ingrid Brück Bøgh; Ida E Holm; Jannik E Jakobsen; Marianne G Johansen; Stig Purup; Lars Bolund; Gábor Vajta; Arne Lund Jørgensen
Journal:  Transgenic Res       Date:  2009-01-29       Impact factor: 2.788

7.  Production of hand-made cloned buffalo (Bubalus bubalis) embryos from non-viable somatic cells.

Authors:  E K A Duah; S K Mohapatra; T J Sood; A Sandhu; S K Singla; M S Chauhan; R S Manik; P Palta
Journal:  In Vitro Cell Dev Biol Anim       Date:  2016-07-15       Impact factor: 2.416

8.  Constitutive expression of the embryonic stem cell marker OCT4 in bovine somatic donor cells influences blastocysts rate and quality after nucleus transfer.

Authors:  Lleretny Rodríguez-Alvarez; Jose Manriquez; Alejandra Velasquez; Fidel Ovidio Castro
Journal:  In Vitro Cell Dev Biol Anim       Date:  2013-07-12       Impact factor: 2.416

Review 9.  Interspecies somatic cell nuclear transfer and preliminary data for horse-cow/mouse iSCNT.

Authors:  R Tayfur Tecirlioglu; Jitong Guo; Alan O Trounson
Journal:  Stem Cell Rev       Date:  2006       Impact factor: 5.739

Review 10.  Stem cell therapies and benefaction of somatic cell nuclear transfer cloning in COVID-19 era.

Authors:  Birbal Singh; Gorakh Mal; Vinod Verma; Ruchi Tiwari; Muhammad Imran Khan; Ranjan K Mohapatra; Saikat Mitra; Salem A Alyami; Talha Bin Emran; Kuldeep Dhama; Mohammad Ali Moni
Journal:  Stem Cell Res Ther       Date:  2021-05-12       Impact factor: 6.832
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