Literature DB >> 11895995

Gel shift assay of nuclear extracts from Histoplasma capsulatum demonstrates the presence of several DNA binding proteins.

Atanas Ignatov1, Elizabeth J Keath.   

Abstract

A gel shift assay was optimized to detect several general DNA binding proteins from Histoplasma capsulatum strain G217B. The electrophoretic mobility shift assay (EMSA) technique also detected protein(s) recognizing a pyrimidine-rich motif found in several Histoplasma promoters. Establishment of EMSA conditions provides an important framework to evaluate regulation of homeostatic or phase-specific genes that may influence virulence in Histoplasma and other dimorphic fungal pathogens.

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Year:  2002        PMID： 11895995      PMCID： PMC127865          DOI： 10.1128/IAI.70.4.2238-2241.2002

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  19 in total

Review 1.  Immune response to early and late Histoplasma capsulatum infections.

Authors:  G S Deepe
Journal:  Curr Opin Microbiol       Date:  2000-08       Impact factor: 7.934

Review 2.  NF-kappa B: a pleiotropic mediator of inducible and tissue-specific gene control.

Authors:  M J Lenardo; D Baltimore
Journal:  Cell       Date:  1989-07-28       Impact factor: 41.582

3.  A dictionary of transcription control sequences.

Authors:  J Locker; G Buzard
Journal:  DNA Seq       Date:  1990

4.  MatInd and MatInspector: new fast and versatile tools for detection of consensus matches in nucleotide sequence data.

Authors:  K Quandt; K Frech; H Karas; E Wingender; T Werner
Journal:  Nucleic Acids Res       Date:  1995-12-11       Impact factor: 16.971

5.  Selective expression of the virulence factor BAD1 upon morphogenesis to the pathogenic yeast form of Blastomyces dermatitidis: evidence for transcriptional regulation by a conserved mechanism.

Authors:  P J Rooney; T D Sullivan; B S Klein
Journal:  Mol Microbiol       Date:  2001-02       Impact factor: 3.501

6.  CAMP is involved in transcriptional regulation of delta9-desaturase during Histoplasma capsulatum morphogenesis.

Authors:  A Storlazzi; B Maresca; S Gargano
Journal:  Mol Cell Biol Res Commun       Date:  1999 Sep-Dec

Review 7.  Molecular epidemiology, pathogenesis, and genetics of the dimorphic fungus Histoplasma capsulatum.

Authors:  D M Retallack; J P Woods
Journal:  Microbes Infect       Date:  1999-08       Impact factor: 2.700

8.  Intracellular parasitism by Histoplasma capsulatum: fungal virulence and calcium dependence.

Authors:  T S Sebghati; J T Engle; W E Goldman
Journal:  Science       Date:  2000-11-17       Impact factor: 47.728

9.  Probing the yeast phase-specific expression of the CBP1 gene in Histoplasma capsulatum.

Authors:  J B Patel; J W Batanghari; W E Goldman
Journal:  J Bacteriol       Date:  1998-04       Impact factor: 3.490

10.  Identification and characterization of a phase-specific, nuclear DNA binding protein from the dimorphic pathogenic fungus Histoplasma capsulatum.

Authors:  F E Abidi; H Roh; E J Keath
Journal:  Infect Immun       Date:  1998-08       Impact factor: 3.441
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  2 in total

1.  Identification of a minimal cis-element and cognate trans-factor(s) required for induction of Rac2 gene expression during K562 cell differentiation.

Authors:  Rajarajeswari Muthukrishnan; David G Skalnik
Journal:  Gene       Date:  2009-04-17       Impact factor: 3.688

2.  Sequence elements necessary for transcriptional activation of BAD1 in the yeast phase of Blastomyces dermatitidis.

Authors:  Peggy J Rooney; Bruce S Klein
Journal:  Eukaryot Cell       Date:  2004-06
  2 in total

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