Literature DB >> 11894901

The DNA-protein cross: a method for detecting specific DNA-protein complexes in crude mixtures.

S B Patel1, P R Cook.   

Abstract

A method using crude cellular mixtures is described which permits identification of polypeptides and DNA fragments forming specific complexes. Our procedure incorporates elements of both 'Southern' and 'protein' blotting and combines, in two dimensions, the resolving power of a denaturing protein gel with that of an agarose DNA gel. Conditions for 'crossing' have been established using the lambda repressor-operator system: the specific complex can be detected by crossing total protein from bacteria overproducing the repressor with a mixture of total genomic fragments from a lysogen.

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Year:  1983        PMID： 11894901      PMCID： PMC555101          DOI： 10.1002/j.1460-2075.1983.tb01395.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  37 in total

Review 1.  Repressors.

Authors:  S Bourgeois; M Pfahl
Journal:  Adv Protein Chem       Date:  1976

2.  The N-terminal amino-acid sequences of DNA polymerase I from Escherichia coli and of the large and the small fragments obtained by a limited proteolysis.

Authors:  H Jacobsen; H Klenow; K Overgaard-Hansen
Journal:  Eur J Biochem       Date:  1974-06-15

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 4.  lambda Repressor and cro--components of an efficient molecular switch.

Authors:  A D Johnson; A R Poteete; G Lauer; R T Sauer; G K Ackers; M Ptashne
Journal:  Nature       Date:  1981-11-19       Impact factor: 49.962

5.  Isolation of high-molecular-weight DNA from mammalian cells.

Authors:  M Gross-Bellard; P Oudet; P Chambon
Journal:  Eur J Biochem       Date:  1973-07-02

6.  A membrane-filter technique for the detection of complementary DNA.

Authors:  D T Denhardt
Journal:  Biochem Biophys Res Commun       Date:  1966-06-13       Impact factor: 3.575

7.  Interactions between DNA-bound repressors govern regulation by the lambda phage repressor.

Authors:  A D Johnson; B J Meyer; M Ptashne
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

8.  Protein component from Drosophila larval nuclei showing sequence specificity for a short region near a major heat-shock protein gene.

Authors:  R S Jack; W J Gehring; C Brack
Journal:  Cell       Date:  1981-05       Impact factor: 41.582

9.  Two heat-resistant, low molecular weight proteins from Escherichia coli that stimulate DNA-directed RNA synthesis.

Authors:  R Cukier-Kahn; M Jacquet; F Gros
Journal:  Proc Natl Acad Sci U S A       Date:  1972-12       Impact factor: 11.205

10.  A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases.

Authors:  N Sigal; H Delius; T Kornberg; M L Gefter; B Alberts
Journal:  Proc Natl Acad Sci U S A       Date:  1972-12       Impact factor: 11.205
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  4 in total

1.  Identification of novel single-stranded d(TC)n binding proteins in several mammalian species.

Authors:  H A Yee; A K Wong; J H van de Sande; J B Rattner
Journal:  Nucleic Acids Res       Date:  1991-02-25       Impact factor: 16.971

2.  Characterization of cis-acting elements regulating transcription of the human DF3 breast carcinoma-associated antigen (MUC1) gene.

Authors:  M Abe; D Kufe
Journal:  Proc Natl Acad Sci U S A       Date:  1993-01-01       Impact factor: 11.205

3.  Detection of a novel minisatellite-specific DNA-binding protein.

Authors:  A Collick; A J Jeffreys
Journal:  Nucleic Acids Res       Date:  1990-02-11       Impact factor: 16.971

4.  Transcriptional activity of the homopurine-homopyrimidine repeat of the c-Ki-ras promoter is independent of its H-forming potential.

Authors:  G Raghu; S Tevosian; S Anant; K N Subramanian; D L George; S M Mirkin
Journal:  Nucleic Acids Res       Date:  1994-08-25       Impact factor: 16.971
  4 in total

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