Literature DB >> 11891706

Microchip-based immunoassay system with branching multichannels for simultaneous determination of interferon-gamma.

Kiichi Sato1, Maho Yamanaka, Hiroko Takahashi, Manabu Tokeshi, Hiroko Kimura, Takehiko Kitamori.   

Abstract

A bead-bed immunoassay system suitable for simultaneous assay of multiple samples was constructed on a microchip. The chip had branching multichannels and four reaction and detection regions; the constructed system could process four samples at a time with only one pump unit. Interferon gamma was assayed by a 3-step sandwich immunoassay with the system coupled to a thermal lens microscope as a detector. The biases of the signal intensities obtained from each channel were within 10%, and coefficients of variation were almost the same level as the single straight channel assay. The assay time for four samples was 50 min instead of 35 min for one sample in the single-channel assay; hence higher throughput was realized with the branching structure chip.

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Year:  2002        PMID: 11891706     DOI: 10.1002/1522-2683(200203)23:5<734::AID-ELPS734>3.0.CO;2-W

Source DB:  PubMed          Journal:  Electrophoresis        ISSN: 0173-0835            Impact factor:   3.535


  17 in total

1.  On-chip antibody immobilization for on-demand and rapid immunoassay on a microfluidic chip.

Authors:  Toshinori Ohashi; Kazuma Mawatari; Takehiko Kitamori
Journal:  Biomicrofluidics       Date:  2010-09-30       Impact factor: 2.800

Review 2.  Protein immobilization techniques for microfluidic assays.

Authors:  Dohyun Kim; Amy E Herr
Journal:  Biomicrofluidics       Date:  2013-07-30       Impact factor: 2.800

3.  Microfluidic flow cytometry: The role of microfabrication methodologies, performance and functional specification.

Authors:  Anil B Shrirao; Zachary Fritz; Eric M Novik; Gabriel M Yarmush; Rene S Schloss; Jeffrey D Zahn; Martin L Yarmush
Journal:  Technology (Singap World Sci)       Date:  2018-03-16

4.  Development and validation of a microfluidic immunoassay capable of multiplexing parallel samples in microliter volumes.

Authors:  Mehdi Ghodbane; Elizabeth C Stucky; Tim J Maguire; Rene S Schloss; David I Shreiber; Jeffrey D Zahn; Martin L Yarmush
Journal:  Lab Chip       Date:  2015-08-07       Impact factor: 6.799

5.  A helical flow, circular microreactor for separating and enriching "smart" polymer-antibody capture reagents.

Authors:  John M Hoffman; Mitsuhiro Ebara; James J Lai; Allan S Hoffman; Albert Folch; Patrick S Stayton
Journal:  Lab Chip       Date:  2010-09-30       Impact factor: 6.799

6.  Development of a low-volume, highly sensitive microimmunoassay using computational fluid dynamics-driven multiobjective optimization.

Authors:  Mehdi Ghodbane; Anthony Kulesa; Henry H Yu; Tim J Maguire; Rene R Schloss; Rohit Ramachandran; Jeffrey D Zahn; Martin L Yarmush
Journal:  Microfluid Nanofluidics       Date:  2015-02       Impact factor: 2.529

7.  Increasing the sensitivity of enzyme-linked immunosorbent assay using multiplexed electrokinetic concentrator.

Authors:  Lih Feng Cheow; Sung Hee Ko; Sung Jae Kim; Kwan Hyoung Kang; Jongyoon Han
Journal:  Anal Chem       Date:  2010-04-15       Impact factor: 6.986

8.  Pulsating bead-based assay.

Authors:  Jason A Thompson; Haim H Bau
Journal:  Anal Chem       Date:  2011-03-28       Impact factor: 6.986

9.  Microfluidic, bead-based assay: Theory and experiments.

Authors:  Jason A Thompson; Haim H Bau
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2009-09-04       Impact factor: 3.205

10.  Heterogeneous immunoassays using magnetic beads on a digital microfluidic platform.

Authors:  Ramakrishna S Sista; Allen E Eckhardt; Vijay Srinivasan; Michael G Pollack; Srinivas Palanki; Vamsee K Pamula
Journal:  Lab Chip       Date:  2008-10-14       Impact factor: 6.799

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