Literature DB >> 118838

Conglutinin binding polyethylene glycol precipitation assay for immune complexes.

M Macanovic, P J Lachmann.   

Abstract

An assay for circulating immune complexes is described which uses radiolabelled bovine conglutinin as ligand and polyethylene glycol precipitation as the technique for separating bound ligands. The technique is simple to perform and gives good sensitivity detecting artificial immune complexes. Its use in detecting complexes in systemic lupus erythematosus and Burkitt's lymphoma is described and it is compared with the Clq binding assay also performed with polyethylene glycol. It is suggested that the simultaneous performance of polyethylene glycol assays using radiolabelled Clq and radiolabelled conglutinin may be an advantageous method for screening sera for the presence of immune complexes.

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Year:  1979        PMID： 118838      PMCID： PMC1537875     

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  7 in total

1.  Preparation of iodine-131 labelled human growth hormone of high specific activity.

Authors:  W M HUNTER; F C GREENWOOD
Journal:  Nature       Date:  1962-05-05       Impact factor: 49.962

2.  Routine assay for the detection of immune complexes of known immunoglobulin class using solid phase C1q.

Authors:  F C Hay; L J Nineham; I M Roitt
Journal:  Clin Exp Immunol       Date:  1976-06       Impact factor: 4.330

3.  Solid-phase enzyme immunoassay or radioimmunoassay for the detection of immune complexes based on their recognition by conglutinin: conglutinin-binding test. A comparative study with 125I-labelled Clq binding and Raji-cell RIA tests.

Authors:  P Casali; A Bossus; N A Carpentier; P H Lambert
Journal:  Clin Exp Immunol       Date:  1977-08       Impact factor: 4.330

4.  Detection of antibodies and soluble antigen-antibody complexes by precipitation with polyethylene glycol.

Authors:  W D Creighton; P H Lambert; P A Miescher
Journal:  J Immunol       Date:  1973-10       Impact factor: 5.422

5.  The preparation and properties of alexinated intermediates that react with conglutinin. II. Equine, rabbit and human complement.

Authors:  P J Lachmann; R Liske
Journal:  Immunology       Date:  1966-09       Impact factor: 7.397

6.  An enzymic method for the trace iodination of immunoglobulins and other proteins.

Authors:  J J Marchalonis
Journal:  Biochem J       Date:  1969-06       Impact factor: 3.857

7.  Activation of the alternative complement pathway due to resistance of zymosan-bound amplification convertase to endogenous regulatory mechanisms.

Authors:  D T Fearon; K F Austen
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205
  7 in total
  18 in total

1.  Reduced complement-mediated immune complex solubilization in leprosy patients.

Authors:  V D Ramanathan; P Sharma; G Ramu; U Sengupta
Journal:  Clin Exp Immunol       Date:  1985-06       Impact factor: 4.330

Review 2.  Immune complexes in human diseases: a review.

Authors:  A N Theofilopoulos; F J Dixon
Journal:  Am J Pathol       Date:  1980-08       Impact factor: 4.307

3.  The isolation of the antibody moieties of immune complexes from serum by the pepsin digestion of conglutinin-anti-conglutinin complexes.

Authors:  P J Lachmann; M Macanovic; G D Harkiss; R G Oldroyd; J Habicht
Journal:  Clin Exp Immunol       Date:  1981-11       Impact factor: 4.330

4.  Serial analysis of circulating immune complexes, complement, and antithymocyte globulin antibodies in heart transplant recipients.

Authors:  G D Harkiss; D L Brown; R Cory-Pearce; T A English
Journal:  J Clin Immunol       Date:  1983-04       Impact factor: 8.317

5.  The effect of a protein meal on three fluid-phase assays for circulating immune complexes.

Authors:  K B Elkon; J G Lanham; A C Dash; G R Hughes
Journal:  Clin Exp Immunol       Date:  1981-08       Impact factor: 4.330

6.  Circulating immune complexes in polymyalgia rheumatica and giant cell arteritis.

Authors:  J R Park; J G Jones; G D Harkiss; B L Hazleman
Journal:  Ann Rheum Dis       Date:  1981-08       Impact factor: 19.103

7.  Differential precipitation of the Clq subcomponent of the first complement component (C1) by polyethylene glycol from normal human serum and sera of patients with collagen diseases.

Authors:  H Van Dijk; J Van Voorst; I G Jankowski; J W Imhof; J A Kerckhaert
Journal:  Clin Exp Immunol       Date:  1984-08       Impact factor: 4.330

8.  Immune complex-like activity associated with abnormal serum lipoproteins in systemic erythematosus.

Authors:  G W Smith; S F Hannan; P J Scott; I J Simpson
Journal:  Clin Exp Immunol       Date:  1982-04       Impact factor: 4.330

9.  Isolation of circulating immune complexes by conglutinin and separation of antigen from dissociated complexes by immobilized protein A.

Authors:  R C Gupta; E M Tan
Journal:  Clin Exp Immunol       Date:  1981-10       Impact factor: 4.330

10.  Evaluation of six tests for circulating IgG complexes with special reference to IgM rheumatoid factors: analysis of systemic lupus erythematosus and rheumatoid arthritis series.

Authors:  O Wager; P Lindström; J A Räsänen; R Kekomäki; B Ziola; A Salmi; H Isomäki; B Skrifvars; K Penttinen
Journal:  Clin Exp Immunol       Date:  1981-10       Impact factor: 4.330
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