Literature DB >> 1188358

Guanidinium-CsCl density gradients for isopycnic analysis of nucleic acids.

V Enea, N D Zinder.   

Abstract

The addition of guanidinium chloride to CsCl gradients lowers the apparent density of RNA, DNA, and hybrid polymers in such a way that all three can be banded, fractionated, and analyzed in one gradient with essentially no damage to their chemical integrity or to their biological activity.

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Year:  1975        PMID: 1188358     DOI: 10.1126/science.1188358

Source DB:  PubMed          Journal:  Science        ISSN: 0036-8075            Impact factor:   47.728


  13 in total

1.  The properties of native and denatured DNA in buoyant rubidium trichloroacetate at neutral pH.

Authors:  R L Burke; W R Bauer
Journal:  Nucleic Acids Res       Date:  1977-06       Impact factor: 16.971

2.  An electron microscope study of the proteins attached to polio virus RNA and its replicative form (RF).

Authors:  M Wu; N Davidson; E Wimmer
Journal:  Nucleic Acids Res       Date:  1978-12       Impact factor: 16.971

3.  Sequence-specific recognition of double helical nucleic acids by proteins.

Authors:  N C Seeman; J M Rosenberg; A Rich
Journal:  Proc Natl Acad Sci U S A       Date:  1976-03       Impact factor: 11.205

4.  Synthesis of a 600-nucleotide-long plus-strand DNA by virions of Moloney murine leukemia virus.

Authors:  S W Mitra; S Goff; E Gilboa; D Baltimore
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  cDNA cloning of a serotonin 5-HT1C receptor by electrophysiological assays of mRNA-injected Xenopus oocytes.

Authors:  H Lübbert; B J Hoffman; T P Snutch; T van Dyke; A J Levine; P R Hartig; H A Lester; N Davidson
Journal:  Proc Natl Acad Sci U S A       Date:  1987-06       Impact factor: 11.205

6.  Translocation affects normal c-myc promoter usage and activates fifteen cryptic c-myc transcription starts in plasmacytoma M603.

Authors:  J Prehn; M Mercola; K Calame
Journal:  Nucleic Acids Res       Date:  1984-12-11       Impact factor: 16.971

7.  Reverse transcription of avian myeloblastosis virus 35S RNA. Early synthesis of plus strand DNA of discrete size in reconstructed reactions.

Authors:  J C Olsen; K F Watson
Journal:  Nucleic Acids Res       Date:  1982-02-11       Impact factor: 16.971

8.  Structure of the provirus within NIH 3T3 cells transfected with Harvey sarcoma virus DNA.

Authors:  M P Goldfarb; R A Weinberg
Journal:  J Virol       Date:  1981-04       Impact factor: 5.103

9.  Isolation and characterization of calmodulin genes from Xenopus laevis.

Authors:  Y H Chien; I B Dawid
Journal:  Mol Cell Biol       Date:  1984-03       Impact factor: 4.272

10.  Propagation of restriction fragments from the mitochondrial DNA of Saccharomyces cerevisiae in E. coli by means of plasmid vectors.

Authors:  P E Berg; A Lewin; T Christianson; M Rabinowitz
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971

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