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Literature DB >> 1187349

Isolation and in vitro translation of human placental lactogen messenger RNA from human term placenta.

Abstract

A messenger activity for HPL was identified in normal human term placentas. The mRNA was translated in rabbit reticulocyte cell-free system. The HPL synthesized was quantified by a specific immunoprecipitation and further identified by electrophoresis on sodium dodecyl sulfate polyacrylamide gel. The HPL synthesized in the reticulocyte lysate exhibited a molecular weight between 20,000 and 22,000 daltons similar to the active hormone. The messenger RNA activity for HPL corresponded to a sedimentation coefficient of 11-12 S. Furthermore the messenger activity for HPL was preferentially associated with membrane bound polyribosomes than with free polyribosomes.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1975 PMID： 1187349 PMCID： PMC343555 DOI： 10.1093/nar/2.10.1903

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

16 in total

1. The translation of a human placental lactogen mRNA fraction in heterologous cell-free systems: the synthesis of a possible precursor.

Authors: I Boime; S Boguslawski; J Caine
Journal: Biochem Biophys Res Commun Date: 1975-01-06 Impact factor: 3.575

2. Protein synthesis in a cell-free system prepared from human placenta. II. pH 5 enzyme inefficiency due to defects in tRNA charging with resulting loss of elongation factor 1.

Authors: C Hubert; B S Baliga; C A Villee; H N Munro
Journal: Biochim Biophys Acta Date: 1974-12-20

3. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.

Authors: R D Palmiter
Journal: Biochemistry Date: 1974-08-13 Impact factor: 3.162

4. Quantitative measurement of ovalbumin messenger ribonucleic acid activity. Localization in polysomes, induction by estrogen, and effect of actinomycin D.

Authors: R E Rhoads; G S McKnight; R T Schimke
Journal: J Biol Chem Date: 1973-03-25 Impact factor: 5.157

5. The molecular properties of human chorionic somatomammotropin.

Authors: S Aloj; H Edelhoch
Journal: J Biol Chem Date: 1971-08-25 Impact factor: 5.157

6. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.

Authors: S Y Lee; J Mendecki; G Brawerman
Journal: Proc Natl Acad Sci U S A Date: 1971-06 Impact factor: 11.205

7. Serum placental lactogen (HPL) levels as an index of placental function.

Authors: B N Saxena; K Emerson; H A Selenkow
Journal: N Engl J Med Date: 1969-07-31 Impact factor: 91.245

8. The effectiveness of human placental lactogen measurements as an adjunct in decreasing perinatal deaths. Results of a retrospective and a randomized controlled prospective study.

Authors: W N Spellacy; W C Buhi; S A Birk
Journal: Am J Obstet Gynecol Date: 1975-03-15 Impact factor: 8.661

Isolation and in vitro translation of human placental lactogen messenger RNA from human term placenta.

1. The translation of a human placental lactogen mRNA fraction in heterologous cell-free systems: the synthesis of a possible precursor.

2. Protein synthesis in a cell-free system prepared from human placenta. II. pH 5 enzyme inefficiency due to defects in tRNA charging with resulting loss of elongation factor 1.

3. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.

4. Quantitative measurement of ovalbumin messenger ribonucleic acid activity. Localization in polysomes, induction by estrogen, and effect of actinomycin D.

5. The molecular properties of human chorionic somatomammotropin.

6. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.

7. Serum placental lactogen (HPL) levels as an index of placental function.

8. The effectiveness of human placental lactogen measurements as an adjunct in decreasing perinatal deaths. Results of a retrospective and a randomized controlled prospective study.

9. The synthesis of human placental lactogen by human placental ribosomes.

10. Isolation and properties of polysomes from human placenta.

1. The synthesis of human placental lactogen hormone (hPL) in a cell-free wheat germ system.

2. Translation in a cell-free system of mRNA from term placenta extracted by guanidine hydrochloride.

3. Synthesis of human placental lactogen in Xenopus oocytes.