Literature DB >> 11858902

A real-time PCR assay for the detection of Neisseria gonorrhoeae by LightCycler.

David M Whiley1, Genevera M LeCornec, Ian M Mackay, David J Siebert, Theo P Sloots.   

Abstract

The detection of Neisseria gonorrhoeae by the polymerase chain reaction (PCR) is now recognized as a sensitive and specific method of diagnosing infection by the organism. In this Study 152 urine specimens were examined for N. gonorrhoeae by a real-time PCR method using the LightCycler platform and results were compared to an "in-house" PCR assay using an ELISA-based detection method. N. gonorrhoeae DNA was detected in 29 (19%) specimens by LightCycler PCR (LC-PCR) and in 31 (20%) specimens by the "in house" PCR method. The LightCycler assay proved to be specific and 94% sensitive when compared to the "in house" PCR method. These features combined with the rapid turn-around time for results makes the LC-PCR particularly suitable for the detection of N. gonorrhoeae in a routine clinical laboratory.

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Year:  2002        PMID： 11858902     DOI： 10.1016/s0732-8893(01)00326-1

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  11 in total

Review 1.  Nucleic acid amplification testing for Neisseria gonorrhoeae: an ongoing challenge.

Authors:  David M Whiley; John W Tapsall; Theo P Sloots
Journal:  J Mol Diagn       Date:  2006-02       Impact factor: 5.568

2.  Diagnostic value of molecular confirmation assays for Neisseria gonorrhoeae.

Authors:  Silke Peter-Getzlaff; Jacqueline Luethy; Burkhard Springer
Journal:  J Clin Microbiol       Date:  2007-11       Impact factor: 5.948

3.  The laboratory diagnosis of Neisseria gonorrhoeae.

Authors:  Lai-King Ng; Irene E Martin
Journal:  Can J Infect Dis Med Microbiol       Date:  2005-01       Impact factor: 2.471

4.  A fast real-time polymerase chain reaction method for sensitive and specific detection of the Neisseria gonorrhoeae porA pseudogene.

Authors:  Stig Ove Hjelmevoll; Merethe Elise Olsen; Johanna U Ericson Sollid; Håkon Haaheim; Magnus Unemo; Vegard Skogen
Journal:  J Mol Diagn       Date:  2006-11       Impact factor: 5.568

5.  Direct comparison of the BD ProbeTec ET system with in-house LightCycler PCR assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae from clinical specimens.

Authors:  Michael G Koenig; Sandra L Kosha; Brandon L Doty; David G Heath
Journal:  J Clin Microbiol       Date:  2004-12       Impact factor: 5.948

6.  Evaluation of conventional and real-time PCR assays using two targets for confirmation of results of the COBAS AMPLICOR Chlamydia trachomatis/Neisseria gonorrhoeae test for detection of Neisseria gonorrhoeae in clinical samples.

Authors:  C H E Boel; C M C van Herk; P J M Berretty; G H W Onland; A J C van den Brule
Journal:  J Clin Microbiol       Date:  2005-05       Impact factor: 5.948

7.  A new confirmatory Neisseria gonorrhoeae real-time PCR assay targeting the porA pseudogene.

Authors:  D M Whiley; P J Buda; J Bayliss; L Cover; J Bates; T P Sloots
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2004-07-10       Impact factor: 3.267

8.  Application of real-time polymerase chain reaction technology to detect prostatic bacteria in patients with chronic prostatitis/chronic pelvic pain syndrome.

Authors:  Satoshi Takahashi; Donald E Riley; John N Krieger
Journal:  World J Urol       Date:  2003-05-13       Impact factor: 4.226

9.  Diagnosis of typhoid fever by polymerase chain reaction.

Authors:  S R Ambati; Gopal Nath; B K Das
Journal:  Indian J Pediatr       Date:  2007-10       Impact factor: 1.967

10.  Neisseria species identification assay for the confirmation of Neisseria gonorrhoeae-positive results of the COBAS Amplicor PCR.

Authors:  Kathy A Mangold; MaryAnn Regner; Mohammed Tajuddin; Aamair M Tajuddin; Lawrence Jennings; Hongyan Du; Karen L Kaul
Journal:  J Clin Microbiol       Date:  2007-03-14       Impact factor: 5.948
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