Literature DB >> 11850446

Simple method for reduction of autofluorescence in fluorescence microscopy.

Michael Neumann1, Detlef Gabel.   

Abstract

Autofluorescence of aldehyde-fixed neural tissue often complicates the use of fluorescence microscopy. Background fluorescence can be notably reduced or eliminated by irradiation with light before treatment with fluorescence probes, resulting in a higher contrast without adversely affecting the staining probabilities.

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Year:  2002        PMID： 11850446     DOI： 10.1177/002215540205000315

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  41 in total

1.  A multispectral LED array for the reduction of background autofluorescence in brain tissue.

Authors:  Haison Duong; Martin Han
Journal:  J Neurosci Methods       Date:  2013-08-27       Impact factor: 2.390

2.  Fluorescence in situ hybridization using peptide nucleic acid probes for rapid detection of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis in potable-water biofilms.

Authors:  Markku J Lehtola; Eila Torvinen; Ilkka T Miettinen; C William Keevil
Journal:  Appl Environ Microbiol       Date:  2006-01       Impact factor: 4.792

Review 3.  Fixation strategies for retinal immunohistochemistry.

Authors:  Tyler W Stradleigh; Andrew T Ishida
Journal:  Prog Retin Eye Res       Date:  2015-04-17       Impact factor: 21.198

4.  Fluorescence imaging preparation methods for tissue scaffolds implanted into a green fluorescent protein porcine model.

Authors:  Sarah E Smith; Richard A White; David A Grant; Sheila A Grant
Journal:  Transgenic Res       Date:  2015-06-25       Impact factor: 2.788

Review 5.  Tutorial: practical considerations for tissue clearing and imaging.

Authors:  Kurt R Weiss; Fabian F Voigt; Douglas P Shepherd; Jan Huisken
Journal:  Nat Protoc       Date:  2021-05-21       Impact factor: 13.491

6.  In focus in HCB.

Authors:  Douglas J Taatjes; Jürgen Roth
Journal:  Histochem Cell Biol       Date:  2017-04-29       Impact factor: 4.304

7.  The metastasis-promoting S100A4 protein confers neuroprotection in brain injury.

Authors:  Oksana Dmytriyeva; Stanislava Pankratova; Sylwia Owczarek; Katrin Sonn; Vladislav Soroka; Christina M Ridley; Alexander Marsolais; Marcos Lopez-Hoyos; Noona Ambartsumian; Eugene Lukanidin; Elisabeth Bock; Vladimir Berezin; Darya Kiryushko
Journal:  Nat Commun       Date:  2012       Impact factor: 14.919

8.  Superresolution intrinsic fluorescence imaging of chromatin utilizing native, unmodified nucleic acids for contrast.

Authors:  Biqin Dong; Luay M Almassalha; Yolanda Stypula-Cyrus; Ben E Urban; John E Chandler; The-Quyen Nguyen; Cheng Sun; Hao F Zhang; Vadim Backman
Journal:  Proc Natl Acad Sci U S A       Date:  2016-08-17       Impact factor: 11.205

9.  A Practical Approach to Quantitative Processing and Analysis of Small Biological Structures by Fluorescent Imaging.

Authors:  Crystal M Noller; Maria Boulina; George McNamara; Angela Szeto; Philip M McCabe; Armando J Mendez
Journal:  J Biomol Tech       Date:  2016-05-13

10.  Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.

Authors:  Raja Chouket; Ruikang Zhang; Agnès Pellissier-Tanon; Annie Lemarchand; Agathe Espagne; Thomas Le Saux; Ludovic Jullien
Journal:  Methods Mol Biol       Date:  2021
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