OBJECTIVE: To develop a real-time PCR technique for detection of the insertion/deletion (I/D) polymorphism of angiotensin-converting enzyme (ACE) gene. DESIGN AND METHODS: Three primers were designed for performing real-time PCR in the presence of SYBR Green I as flurochrome followed by melting curve analysis. Forty human genomic DNA that have been genotyped by two-rounds of conventional PCR were used for evaluation of this technique. RESULTS: Melting curve analysis indicated the melting peak at 73.9 degrees C and 76.2 degrees C corresponding to the presence of I and D alleles, respectively. Comparable genotyping results were obtained by both conventional and real-time PCR. Besides, the mistyping of ID allele individuals by the first run of conventional PCR were accurately genotyped by single-tube real time PCR. CONCLUSIONS: The real-time PCR method presented in this study provides a rapid and sensitive way for genotyping of ACE gene that may be suitable for large-scale clinical and epidemiologic study.
OBJECTIVE: To develop a real-time PCR technique for detection of the insertion/deletion (I/D) polymorphism of angiotensin-converting enzyme (ACE) gene. DESIGN AND METHODS: Three primers were designed for performing real-time PCR in the presence of SYBR Green I as flurochrome followed by melting curve analysis. Forty human genomic DNA that have been genotyped by two-rounds of conventional PCR were used for evaluation of this technique. RESULTS: Melting curve analysis indicated the melting peak at 73.9 degrees C and 76.2 degrees C corresponding to the presence of I and D alleles, respectively. Comparable genotyping results were obtained by both conventional and real-time PCR. Besides, the mistyping of ID allele individuals by the first run of conventional PCR were accurately genotyped by single-tube real time PCR. CONCLUSIONS: The real-time PCR method presented in this study provides a rapid and sensitive way for genotyping of ACE gene that may be suitable for large-scale clinical and epidemiologic study.
Authors: Tórur Sjúrðarson; Jacob Bejder; Andreas Breenfeldt Andersen; Thomas Bonne; Kasper Kyhl; Tóra Róin; Poula Patursson; Noomi Oddmarsdóttir Gregersen; May-Britt Skoradal; Michael Schliemann; Malte Lindegaard; Pál Weihe; Magni Mohr; Nikolai B Nordsborg Journal: Physiol Rep Date: 2022-07
Authors: Tórur Sjúrðarson; Jacob Bejder; Andreas Breenfeldt Andersen; Thomas C Bonne; Kasper Kyhl; Martin Thomassen; Júlia Prats; Noomi Oddmarsdóttir Gregersen; May-Britt Skoradal; Pál Weihe; Nikolai B Nordsborg; Magni Mohr Journal: Eur J Appl Physiol Date: 2022-10-22 Impact factor: 3.346
Authors: Daméhan Tchelougou; Jonas K Kologo; Simplice D Karou; Valentin N Yaméogo; Cyrille Bisseye; Florencia W Djigma; Djeneba Ouermi; Tegwindé R Compaoré; Maléki Assih; Virginio Pietra; Patrice Zabsonré; Jacques Simpore Journal: Int J Hypertens Date: 2015-08-17 Impact factor: 2.420