The performance of microparticle chemically-bonded anion-exchange resins in the analysis of nucleotides.

The performance of microparticles in the ion-exchange chromatographic separation of nucleotides was investigated. A microparticle chemically-bonded strong anion-exchange column packing was used for the analysis of the 5'-mono-, di- and -triphosphate nucleotides of adenine, guanine, hypoxanthine, xanthine, cytosine, uracil and thymine. It was found that excellent resolution of the majority of the nucleotides was obtained at ambient temperatures. The packings were stable and the sample capacity was greater than with pellicular resins. The retention times, peak shapes, heights and areas were highly reproducible and there was good linearity of response. Use of this column packing in biochemical studies is reported.