Molecular probes of the vestibular nerve. II. Characterization of neurons in Scarpa's ganglion to determine separate populations within the nerve.

An unambiguous delineation of the exact numbers and/or proportions of calyx-only, dimorph, and bouton-only vestibular afferents is needed to continue studies concerning vestibular integration in the nervous system. Here, we take advantage of immunocytochemical properties of three groups of vestibular afferents. We utilize calretinin to delineate the calyx-only population, and peripherin to stain the bouton-only afferents. An additional subgroup of afferents that stain with calbindin, but not calretinin is also introduced. The size of the cells that stain with these markers was determined. Cells that are calbindin-positive overlap the sizes of Nissl-stained somata. Cells that stain with peripherin or calretinin are non-overlapping with calretinin cells being the largest and peripherin-positive cells the smallest. Twenty percent of the ganglion cells were peripherin positive, another 20% stained with calretinin antibodies, 30% stained with calbindin, and all cells in Scarpa's ganglion stained with parvalbumin. Most of the calretinin-positive cells also stained with calbindin. One-third of the calbindin-positive population stained only with calbindin. These studies indicate that the calyx- and bouton-only populations of vestibular afferents in gerbil comprise at least 40% of the nerve. In addition, at least 10% of the nerve also stains with calbindin and neither calretinin nor peripherin. Based on indirect evidence, we hypothesize that these are a subpopulation of dimorph afferents. This study has provided an anatomical instrument (in addition to intracellular physiological methods) to study separate populations of vestibular afferents.