Studying actin-dependent processes in tissue culture.

The cytoskeletal organization of cells that are grown in tissue culture is often very different from that of cells in living organisms. This casts some doubt as to whether information that comes from studying actin-dependent cellular processes--such as cell motility or differentiation--in cells that are cultured under these conditions is physiologically relevant. Studies on cells grown in improved two-dimensional- and three-dimensional-culture systems that closely mimic the in vivo extracellular-matrix environment should provide a more accurate picture of actin-cytoskeletal function in the living organism.