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Literature DB >> 12668480

Internet-based image analysis quantifies contractile behavior of individual fibroblasts inside model tissue.

Steven Vanni¹, B Christoffer Lagerholm, Carol Otey, D Lansing Taylor, Frederick Lanni.

Abstract

In a cell-populated collagen gel, intrinsic fiber structure visible in differential interference contrast images can provide markers for an in situ strain gauge to quantify cell-gel mechanics, while optical sections of fluorescent protein distribution capture cytoskeletal kinematics. Mechanics quantification can be derived automatically from timelapse differential interference contrast images using a Deformation Quantification and Analysis software package accessible online at http://dqa.web.cmu.edu. In our studies, fibroblast contractile machinery was observed to function entirely within pseudopods, while GFP-alpha-actinin concentrated in pseudopod tips and cortex. Complex strain patterns around individual cells showed instances of both elastic and inelastic strain transmission, suggesting a role in observed long-range alignment of cells.

Entities: Disease

Mesh：

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Year: 2003 PMID： 12668480 PMCID： PMC1302838 DOI： 10.1016/s0006-3495(03)75077-2

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

58 in total

1. Keratocytes generate traction forces in two phases.

Authors: K Burton; J H Park; D L Taylor
Journal: Mol Biol Cell Date: 1999-11 Impact factor: 4.138

2. Effect of cell migration on the maintenance of tension on a collagen matrix.

Authors: P Roy; W M Petroll; C J Chuong; H D Cavanagh; J V Jester
Journal: Ann Biomed Eng Date: 1999 Nov-Dec Impact factor: 3.934

3. Integrin alpha 2 beta 1 (VLA-2) mediates reorganization and contraction of collagen matrices by human cells.

Authors: J A Schiro; B M Chan; W T Roswit; P D Kassner; A P Pentland; M E Hemler; A Z Eisen; T S Kupper
Journal: Cell Date: 1991-10-18 Impact factor: 41.582

4. Regulation of LPA-promoted myofibroblast contraction: role of Rho, myosin light chain kinase, and myosin light chain phosphatase.

Authors: M Parizi; E W Howard; J J Tomasek
Journal: Exp Cell Res Date: 2000-02-01 Impact factor: 3.905

5. Mechanisms and dynamics of mechanical strengthening in ligament-equivalent fibroblast-populated collagen matrices.

Authors: D Huang; T R Chang; A Aggarwal; R C Lee; H P Ehrlich
Journal: Ann Biomed Eng Date: 1993 May-Jun Impact factor: 3.934

6. Increased myosin light chain phosphorylation is not required for growth factor stimulation of collagen matrix contraction.

Authors: G Skuta; C H Ho; F Grinnell
Journal: J Biol Chem Date: 1999-10-15 Impact factor: 5.157

7. Demonstration of a direct role for myosin light chain kinase in fibroblast-populated collagen lattice contraction.

Authors: H P Ehrlich; W B Rockwell; T L Cornwell; J B Rajaratnam
Journal: J Cell Physiol Date: 1991-01 Impact factor: 6.384

Review 8. To do tissue culture in two or three dimensions? That is the question.

Authors: R M Hoffman
Journal: Stem Cells Date: 1993-03 Impact factor: 6.277

9. Isometric contraction by fibroblasts and endothelial cells in tissue culture: a quantitative study.

Authors: M S Kolodney; R B Wysolmerski
Journal: J Cell Biol Date: 1992-04 Impact factor: 10.539

10. Decreased level of PDGF-stimulated receptor autophosphorylation by fibroblasts in mechanically relaxed collagen matrices.

Authors: Y C Lin; F Grinnell
Journal: J Cell Biol Date: 1993-08 Impact factor: 10.539

21 in total

Internet-based image analysis quantifies contractile behavior of individual fibroblasts inside model tissue.

1. Keratocytes generate traction forces in two phases.

2. Effect of cell migration on the maintenance of tension on a collagen matrix.

3. Integrin alpha 2 beta 1 (VLA-2) mediates reorganization and contraction of collagen matrices by human cells.

4. Regulation of LPA-promoted myofibroblast contraction: role of Rho, myosin light chain kinase, and myosin light chain phosphatase.

5. Mechanisms and dynamics of mechanical strengthening in ligament-equivalent fibroblast-populated collagen matrices.

6. Increased myosin light chain phosphorylation is not required for growth factor stimulation of collagen matrix contraction.

7. Demonstration of a direct role for myosin light chain kinase in fibroblast-populated collagen lattice contraction.

Review 8. To do tissue culture in two or three dimensions? That is the question.

9. Isometric contraction by fibroblasts and endothelial cells in tissue culture: a quantitative study.

10. Decreased level of PDGF-stimulated receptor autophosphorylation by fibroblasts in mechanically relaxed collagen matrices.

1. Dynamic imaging of cellular interactions with extracellular matrix.

2. Luminescent nanocrystal stress gauge.

3. Cell-matrix entanglement and mechanical anchorage of fibroblasts in three-dimensional collagen matrices.

4. Three-dimensional modeling of mechanical forces in the extracellular matrix during epithelial lumen formation.

5. Using gold nanorods to probe cell-induced collagen deformation.

6. Mapping local matrix remodeling induced by a migrating tumor cell using three-dimensional multiple-particle tracking.

7. Nonlinear strain stiffening is not sufficient to explain how far cells can feel on fibrous protein gels.

8. Striated acto-myosin fibers can reorganize and register in response to elastic interactions with the matrix.

9. Comparison of cell migration mechanical strategies in three-dimensional matrices: a computational study.

10. Nonlinear Elasticity of the ECM Fibers Facilitates Efficient Intercellular Communication.