Literature DB >> 1182991

More sensitive automated detection of polyamines in physiological fluids and tissue extracts with omicron-phthalaldehyde.

L J Marton, P L Lee.   

Abstract

Omicron-Phthalaldehyde reacts with primary amines to form an intensely blue-fluorescing product. Replacing ninhydrin with this compound in our automated high-pressure liquid-chromatographic technique for determination of polyamines, we achieved a 6- to 10-fold increase in sensitivity. Linearity and reproducibility are excellent, and the method is shown to be applicable to analysis of physiological fluids and of tissue extracts.

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Year:  1975        PMID： 1182991

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  12 in total

1.  An Alkalophilic Bacillus sp. Produces 2-Phenylethylamine.

Authors:  N Hamasaki; S Shirai; M Niitsu; K Kakinuma; T Oshima
Journal:  Appl Environ Microbiol       Date:  1993-08       Impact factor: 4.792

2.  Involvement of the bacterial groM gene product in bacteriophage T7 reproduction. II. A reduced level of ion concentrations causes the blockage of T7 maturation in K-12-M cells.

Authors:  A H Kuhn; H Jütte; E Kellenberger
Journal:  J Virol       Date:  1983-09       Impact factor: 5.103

3.  A simplified method for isocratic HPLC analysis of polyamines.

Authors:  W L Whitmore; T A Slotkin
Journal:  Experientia       Date:  1985-09-15

4.  Spermidine or spermine is essential for the aerobic growth of Saccharomyces cerevisiae.

Authors:  D Balasundaram; C W Tabor; H Tabor
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-01       Impact factor: 11.205

5.  Regulatory interrelations between GABA and polyamines. II. Effect of GABA on ornithine decarboxylase and putrescine levels in cell culture.

Authors:  P P McCann; J M Hornsperger; N Seiler
Journal:  Neurochem Res       Date:  1979-08       Impact factor: 3.996

6.  Regulatory interrelations between GABA and polyamines. I. Brain GABA levels and polyamine metabolism.

Authors:  N Seiler; G Bink; J Grove
Journal:  Neurochem Res       Date:  1979-08       Impact factor: 3.996

7.  Red blood cell polyamines as a diagnostic indicator of glioma presence and recurrence.

Authors:  P Elworthy; E Hitchcock
Journal:  J Neurooncol       Date:  1989-05       Impact factor: 4.130

8.  Productive phage infection in Escherichia coli with reduced internal levels of the major cations.

Authors:  A Kuhn; E Kellenberger
Journal:  J Bacteriol       Date:  1985-09       Impact factor: 3.490

9.  Indirect evidence for a strict negative control of S-adenosyl-L-methionine decarboxylase by spermidine in rat hepatoma cells.

Authors:  P S Mamont; A M Joder-Ohlenbusch; M Nussli; J Grove
Journal:  Biochem J       Date:  1981-05-15       Impact factor: 3.857

10.  Adenovirus type 5 induces progression of quiescent rat cells into S phase without polyamine accumulation.

Authors:  B F Cheetham; D C Shaw; A J Bellett
Journal:  Mol Cell Biol       Date:  1982-10       Impact factor: 4.272
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