Lipolysis and ultrastructural changes of intracellular lipid vesicles after cooling of bovine and porcine GV-oocytes.

The aim of our investigation was to compare the ultrastructure of lipid droplets, and the effect of cooling on intracellular lipid vesicles of bovine and porcine GV oocytes. The lipid droplets in bovine GV oocytes have a homogeneous structure. The utilization of lipids takes place directly from these vesicles without formation of interim lipid compounds. In contrast, there are two kinds of lipid droplets in porcine GV oocytes: 'dark', homogeneous vesicles next to 'grey' vesicles with electron-lucent streaks. Vesicles of each specific group are connected to each other. After a 12-h culture, the formation of the cisternal smooth endoplasmic reticulum layer was always associated with 'grey' lipid vesicles. This is evidence that during oogenesis lipolysis takes place only in 'grey' vesicles. It is supposed that cytoplasmic lipolysis has two stages: 'dark' vesicles change into a 'grey' form followed by a utilization of these 'grey' lipids. Furthermore, both types of lipid droplets in porcine oocytes changed morphologically during cooling: they changed into a spherical form with lucent streaks. Lipid droplets in bovine GV oocytes revealed no visible morphological changes after cooling.