Literature DB >> 11819265

Cloning and expression of NS3 cDNA fragment of HCV genome of Hebei isolate in E.coli.

Fen-Lu Zhu, Hao-Ying Lu, Zhuo Li, Zhong-Tian Qi.   

Abstract

AIM:To obtain greater antigenicity of HCV NS3 protein.
METHODS: The HCV NS3 cDNA fragment was amplified by reverse transcription polymerase chain reaction from the sera of the HCV infected patients.The DNA sequence was determined by dideoxy-mediated chain termination method using T7 polymerase.HCV NS3 protein was expressed in E. coli.
RESULTS: Sequence analysis indicated that the HCV isolate of this study belongs to HCV-II; SDS-PAGE demonstrated an M(r) 23800 and an M(r) 22000 recombinant protein band which amount to 14% and 11% of the total bacterial proteins separately.Western blotting and ELISA showed NS3 protein possessed greater antigenicity.
CONCLUSION: Recombinant HCV NS3 protein was expressed successfully, which provided the basis for developing HCV diagnostic reagents.

Entities:  

Year:  1998        PMID： 11819265      PMCID： PMC4688643          DOI： 10.3748/wjg.v4.i2.165

Source DB:  PubMed          Journal:  World J Gastroenterol        ISSN： 1007-9327            Impact factor:   5.742


  10 in total

1.  Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome.

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2.  Hepatitis C viral RNA in serum of patients with chronic non-A, non-B hepatitis: detection by the polymerase chain reaction using multiple primer sets.

Authors:  K Cristiano; A M Di Bisceglie; J H Hoofnagle; S M Feinstone
Journal:  Hepatology       Date:  1991-07       Impact factor: 17.425

3.  Nucleotide sequence of the genomic RNA of hepatitis C virus isolated from a human carrier: comparison with reported isolates for conserved and divergent regions.

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Journal:  J Gen Virol       Date:  1991-11       Impact factor: 3.891

4.  Hepatitis C virus RNA in blood donor sera detected by the polymerase chain reaction: comparison with supplementary hepatitis C antibody assays.

Authors:  A Widell; A S Månsson; G Sundström; B G Hansson; E Nordenfelt
Journal:  J Med Virol       Date:  1991-12       Impact factor: 2.327

5.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

6.  Structure and organization of the hepatitis C virus genome isolated from human carriers.

Authors:  A Takamizawa; C Mori; I Fuke; S Manabe; S Murakami; J Fujita; E Onishi; T Andoh; I Yoshida; H Okayama
Journal:  J Virol       Date:  1991-03       Impact factor: 5.103

7.  Genetic organization and diversity of the hepatitis C virus.

Authors:  Q L Choo; K H Richman; J H Han; K Berger; C Lee; C Dong; C Gallegos; D Coit; R Medina-Selby; P J Barr
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

8.  Full-length sequence of a hepatitis C virus genome having poor homology to reported isolates: comparative study of four distinct genotypes.

Authors:  H Okamoto; K Kurai; S Okada; K Yamamoto; H Lizuka; T Tanaka; S Fukuda; F Tsuda; S Mishiro
Journal:  Virology       Date:  1992-05       Impact factor: 3.616

9.  The Taiwanese hepatitis C virus genome: sequence determination and mapping the 5' termini of viral genomic and antigenomic RNA.

Authors:  P J Chen; M H Lin; K F Tai; P C Liu; C J Lin; D S Chen
Journal:  Virology       Date:  1992-05       Impact factor: 3.616

10.  New plasmid vectors for high level synthesis of eukaryotic fusion proteins in Escherichia coli.

Authors:  D L Rimm; T D Pollard
Journal:  Gene       Date:  1989-02-20       Impact factor: 3.688
  10 in total
  5 in total

1.  Immunogenicity of HGV NS5 protein expressed from Sf9 insect cells.

Authors:  H Ren; F L Zhu; S Y Zhu; Y Song; Z T Qi
Journal:  World J Gastroenterol       Date:  2001-02       Impact factor: 5.742

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Authors:  Chao-Pin Li; Ke-Xia Wang; Jian Wang; Bo-Rong Pan
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3.  Preparation of human single chain Fv antibody against hepatitis C virus E2 protein and its identification in immunohistochemistry.

Authors:  Yan-Wei Zhong; Jun Cheng; Gang Wang; Shuang-Shuang Shi; Li Li; Ling-Xia Zhang; Ju-Mei Chen
Journal:  World J Gastroenterol       Date:  2002-10       Impact factor: 5.742

4.  Methodologic research on TIMP-1, TIMP-2 detection as a new diagnostic index for hepatic fibrosis and its significance.

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5.  Cloning and expression of NS3 helicase fragment of hepatitis C virus and the study of its immunoreactivity in HCV infected patients.

Authors:  Mahrou Sadri; Mohammad Morad Farajollahi; Zohreh Sharifi
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  5 in total

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