AIM:To offer a more simple method with a high sensitivity and specificity for detection of hepatoma cells in peripheral blood of the patients with HCC. METHODS: Improved nested RT-PCR method was used to detect the expression of AFP mRNA in nuclear cells separated from peripheral venous blood. RESULTS: AFP mRNA contained in tenhepatoma cells was detected from 2mL peripheral blood. CONCLUSION: The improved nested RT-PCR assay for AFP mRNA expressed in cancer cells in peripheral blood might be a valuable method for clinical diagnosis of HCC.
AIM:To offer a more simple method with a high sensitivity and specificity for detection of hepatoma cells in peripheral blood of the patients with HCC. METHODS: Improved nested RT-PCR method was used to detect the expression of AFP mRNA in nuclear cells separated from peripheral venous blood. RESULTS:AFP mRNA contained in tenhepatoma cells was detected from 2mL peripheral blood. CONCLUSION: The improved nested RT-PCR assay for AFP mRNA expressed in cancer cells in peripheral blood might be a valuable method for clinical diagnosis of HCC.
Authors: M Matsumura; Y Niwa; Y Hikiba; K Okano; N Kato; S Shiina; Y Shiratori; M Omata Journal: Biochem Biophys Res Commun Date: 1995-02-15 Impact factor: 3.575
Authors: W S Ji; J L Hu; J W Qiu; B R Pan; D R Peng; B L Shi; S J Zhou; K C Wu; D M Fan Journal: World J Gastroenterol Date: 2001-12 Impact factor: 5.742