Literature DB >> 1181666

A combined radioimmunoassay for glucagon and insulin.

V Leclercq-Meyer, J Marchand, O Rebolledo, W J Malaisse, R Leclercq.   

Abstract

A combined radioimmunoassay for glucagon and insulin in biological fluids is presented. It is based on the use of 131I-glucagon and 125I-insulin tracers and a charcoal-dextran separation procedure. Standard curves, sample determinations and recovery studies gave comparable results whether in the combined or individual assay for glucagon and insulin. The combined assay, especially if supported by a decoding and calculating computer program, offers the advantages that it requires a smaller volume of the material to be sampled, is more economical and less time-consuming.

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Year:  1975        PMID： 1181666     DOI： 10.1007/bf00429910

Source DB:  PubMed          Journal:  Diabetologia        ISSN： 0012-186X            Impact factor:   10.122


  17 in total

1.  Failure of parathormone to affect insulin and glucagon release from the perfused rat pancreas.

Authors:  O Rebolledo; V Leclercq-Meyer; J Marchand; R Leclercq; W J Malaisse
Journal:  Horm Metab Res       Date:  1975-07       Impact factor: 2.936

Review 2.  Alpha- and beta-cell interrelationships in health and disease.

Authors:  R H Unger
Journal:  Metabolism       Date:  1974-06       Impact factor: 8.694

3.  Effect of glucagon: insulin ratios on hepatic metabolism.

Authors:  R Parrilla; M N Goodman; C J Toews
Journal:  Diabetes       Date:  1974-09       Impact factor: 9.461

4.  Radioimmunoassay and saturation analysis. Basic principles and theory.

Authors:  R P Ekins
Journal:  Br Med Bull       Date:  1974-01       Impact factor: 4.291

5.  [A method of radioimmunological determination of glucagon involving separation by charcoal and dextran].

Authors:  V Leclercq-Meyer; P Mialhe; W J Malaisse
Journal:  Diabetologia       Date:  1970-04       Impact factor: 10.122

6.  Coated charcoal immunoassay of insulin.

Authors:  V Herbert; K S Lau; C W Gottlieb; S J Bleicher
Journal:  J Clin Endocrinol Metab       Date:  1965-10       Impact factor: 5.958

7.  Simultaneous assay of insulin and glucagon in serum.

Authors:  J D Young; E W Kraegen
Journal:  Aust J Exp Biol Med Sci       Date:  1968-12

8.  Glucagon: role in the hyperglycemia of diabetes mellitus.

Authors:  R Dobbs; H Sakurai; H Sasaki; G Faloona; I Valverde; D Baetens; L Orci; R Unger
Journal:  Science       Date:  1975-02-14       Impact factor: 47.728

9.  Immunoassay of human insulin and growth hormone simultaneously using I-131 and I-125 tracers.

Authors:  C R Morgan
Journal:  Proc Soc Exp Biol Med       Date:  1966-10

10.  Effects of glucagon on lipolysis and ketogenesis in normal and diabetic men.

Authors:  J E Liljenquist; J D Bomboy; S B Lewis; B C Sinclair-Smith; P W Felts; W W Lacy; O B Crofford; G W Liddle
Journal:  J Clin Invest       Date:  1974-01       Impact factor: 14.808
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  8 in total

1.  Altered anomeric specificity of glucose-induced insulin release in rabbits with duct-ligated pancreas.

Authors:  F Fichaux; J Marchand; B Yaylali; V Leclercq-Meyer; J Catala; W J Malaisse
Journal:  Int J Pancreatol       Date:  1991-02

2.  The role of calcium in glucagon release, interactions between glucose and calcium.

Authors:  V Leclercq-Meyer; J Marchand; W J Malaisse
Journal:  Diabetologia       Date:  1976-10       Impact factor: 10.122

3.  Dynamics of the cationic, bioelectrical and secretory responses to formycin A in pancreatic islet cells.

Authors:  P Lebrun; E Renström; M H Antoine; K Bokvist; M Holmquist; P Rorsman; W J Malaisse
Journal:  Pflugers Arch       Date:  1996-01       Impact factor: 3.657

4.  Role of glucose and insulin in the dynamic regulation of glucagon release by the perfused rat pancreas.

Authors:  V Leclercq-Meyer; J Marchand; W J Malaisse
Journal:  Diabetologia       Date:  1983-03       Impact factor: 10.122

5.  Dynamics of insulin release by perifused insulin-producing tumoral cells: effects of glucose, forskolin, leucine, barium and theophylline.

Authors:  V Leclercq-Meyer; M H Giroix; A Sener; J Marchand; W J Malaisse
Journal:  Int J Pancreatol       Date:  1988 Jan-Feb

6.  Autonomic activity and glycemic homeostasis are maintained by precocious and low intensity training exercises in MSG-programmed obese mice.

Authors:  Dionizia Xavier Scomparin; Rodrigo Mello Gomes; Sabrina Grassiolli; Wilson Rinaldi; Adriana Gallego Martins; Júlio Cezar de Oliveira; Clarice Gravena; Paulo Cezar de Freitas Mathias
Journal:  Endocrine       Date:  2009-10-24       Impact factor: 3.633

7.  Interactions of alpha-ketoisocaproate, glucose and arginine in the secretion of glucagon and insulin from the perfused rat pancreas.

Authors:  V Leclercq-Meyer; J Marchand; R Leclercq; W J Malaisse
Journal:  Diabetologia       Date:  1979-08       Impact factor: 10.122

8.  Evidence for a limited role of NAD(P)H in the nutritional regulation of glucagon release: studies with menadione and NH4Cl.

Authors:  V Leclerq-Meyer; J Marchand; W J Malaisse
Journal:  Acta Diabetol Lat       Date:  1980 Jan-Mar
  8 in total

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