Literature DB >> 1180897

The use of ammonium acetate in the precipitation of ribonucleic acid.

H H Osterburg, J K Allen, C E Finch.   

Abstract

Ammonium acetate (0.24M) in combination with cold 66% (v/v) ethanol quantitatively precipitated RNA from very dilute solutions (greater than or equal to mug/ml) after centrifugation for 2.5 X 10(6)g-min. There was also less co-precipitation of detergents with ammonium acetate than NaCl.

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Year:  1975        PMID： 1180897      PMCID： PMC1165452          DOI： 10.1042/bj1470367

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  5 in total

1.  ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID.

Authors:  K S KIRBY
Journal:  Biochem J       Date:  1965-07       Impact factor: 3.857

2.  Genomic function during the lampbrush chromosome stage of amphibian oogenesis.

Authors:  E H Davidson; M Crippa; F R Kramer; A E Mirsky
Journal:  Proc Natl Acad Sci U S A       Date:  1966-09       Impact factor: 11.205

3.  General interspersion of repetitive with non-repetitive sequence elements in the DNA of Xenopus.

Authors:  E H Davidson; B R Hough; C S Amenson; R J Britten
Journal:  J Mol Biol       Date:  1973-06-15       Impact factor: 5.469

4.  A procedure for the isolation of mammalian messenger ribonucleic acid.

Authors:  G Brawerman; J Mendecki; S Y Lee
Journal:  Biochemistry       Date:  1972-02-15       Impact factor: 3.162

5.  Persistence in early amphibian embryos of informational RNA's from the lampbrush chromosome stage of oögenesis.

Authors:  M Crippa; E H Davidson; A E Mirsky
Journal:  Proc Natl Acad Sci U S A       Date:  1967-04       Impact factor: 11.205
  5 in total
  9 in total

1.  Translation of tyrosine hydroxylase from poly(A)-mRNA in pheochromocytoma cells is enhanced by dexamethasone.

Authors:  E E Baetge; B B Kaplan; D J Reis; T H Joh
Journal:  Proc Natl Acad Sci U S A       Date:  1981-02       Impact factor: 11.205

2.  Isolation of messenger RNA coding for the "fast" protein of embryonic chick feathers.

Authors:  B C Powell; G E Rogers
Journal:  Nucleic Acids Res       Date:  1979-12-20       Impact factor: 16.971

3.  Translational activity of messenger ribonucleic acid isolated from unstimulated and phytohaemagglutinin-activated lymphocytes.

Authors:  D M Wallace; R Jagus; C R Benzie; J E Kay
Journal:  Biochem J       Date:  1979-11-15       Impact factor: 3.857

4.  An improved method for the rapid isolation of brain ribonucleic acid.

Authors:  B B Kaplan; S L Bernstein; A E Gioio
Journal:  Biochem J       Date:  1979-10-01       Impact factor: 3.857

5.  A simple procedure for the isolation and purification of protamine messenger ribonucleic acid from trout testis.

Authors:  L Gedamu; K Iatrou; G H Dixon
Journal:  Biochem J       Date:  1978-06-01       Impact factor: 3.857

6.  Characterization of adenovirus type 2 transcriptional complexes isolated from infected HeLa cell nuclei.

Authors:  J Wilhelm; O Brison; C Kedinger; P Chambon
Journal:  J Virol       Date:  1976-07       Impact factor: 5.103

7.  Determination of tissue-type plasminogen-activator mRNA in human and non-human cell lines by dot-blot hybridization.

Authors:  G Opdenakker; A Billiau; G Volckaert; P de Somer
Journal:  Biochem J       Date:  1985-10-15       Impact factor: 3.857

8.  Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage.

Authors:  Ryan S McCulloch; Melissa S Ashwell; Audrey T O'Nan; Peter L Mente
Journal:  J Anim Sci Biotechnol       Date:  2012-11-12

9.  Nuclear matrix and hnRNP share a common structural constituent associated with premessenger RNA.

Authors:  H Gallinaro; E Puvion; L Kister; M Jacob
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
  9 in total

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