| Literature DB >> 1180895 |
M D Scawen, J A Ramshaw, D Boulter.
Abstract
The amino acid sequence of spinach (Spinacia oleracea L.) plastocyanin was determined. It consists of a single polypeptide chain of 99 residues and has a sequence molecular weight of 10415. The sequence was determined by using a Beckman 890C automatic sequencer and by the dansyl--phenyl isothiocyanate analysis of peptides obtained by the enzymic digestion of purified CNBr fragments. Overlap through the two methionine residues was not shown. Sedimentation equilibrium in the ultracentrifuge gave a molecular weight for spinach plastocyanin of about 9000, in contrast with the value of 21000 reported previously by Katoh et al. (1962).Entities:
Mesh:
Substances:
Year: 1975 PMID: 1180895 PMCID: PMC1165448 DOI: 10.1042/bj1470343
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857