PURPOSE: To compare fertilization and pregnancy rates of fresh and frozen-thawed testicular sperm injections (TESE-ICSI). METHODS: Sperm collected from the testes of 28 azoospermic patients by an open testicular biopsy technique was used for initial ICSI or cryopreserved. RESULTS: Fresh-sperm ICSI treatment (28 cycles) resulted in a 58.1% fertilization rate and a 32.1% clinical pregnancy rate per embryo transfer, while frozen-thawed sperm (24 subsequent cycles) had rates of 54.5 and 29.2%, respectively. The PR was lower using frozen-thawed sperm from nonobstructive azoospermia patients (9.1%) than from obstructive azoospermia patients (46.2%). PR declined to 0% upon the fourth ICSI attempt. CONCLUSIONS: Fertilization, embryo cleavage, and pregnancy rates were unaffected by fresh or frozen-thawed sperm use. A 57.1% cumulative clinical PR was achieved using the latter. The PR was significantly lower using frozen-thawed sperm from nonobstructive azoospermia patients than from obstructive azoospermia patients.
PURPOSE: To compare fertilization and pregnancy rates of fresh and frozen-thawed testicular sperm injections (TESE-ICSI). METHODS: Sperm collected from the testes of 28 azoospermic patients by an open testicular biopsy technique was used for initial ICSI or cryopreserved. RESULTS: Fresh-sperm ICSI treatment (28 cycles) resulted in a 58.1% fertilization rate and a 32.1% clinical pregnancy rate per embryo transfer, while frozen-thawed sperm (24 subsequent cycles) had rates of 54.5 and 29.2%, respectively. The PR was lower using frozen-thawed sperm from nonobstructive azoospermia patients (9.1%) than from obstructive azoospermiapatients (46.2%). PR declined to 0% upon the fourth ICSI attempt. CONCLUSIONS: Fertilization, embryo cleavage, and pregnancy rates were unaffected by fresh or frozen-thawed sperm use. A 57.1% cumulative clinical PR was achieved using the latter. The PR was significantly lower using frozen-thawed sperm from nonobstructive azoospermia patients than from obstructive azoospermiapatients.
Authors: G D Palermo; P N Schlegel; J J Hariprashad; B Ergün; A Mielnik; N Zaninovic; L L Veeck; Z Rosenwaks Journal: Hum Reprod Date: 1999-03 Impact factor: 6.918
Authors: W Küpker; P N Schlegel; S Al-Hasani; P Fornara; R Johannisson; J Sandmann; T Schill; M Bals-Pratsch; M Ludwig; K Diedrich Journal: Fertil Steril Date: 2000-03 Impact factor: 7.329
Authors: S J Silber; Z Nagy; J Liu; H Tournaye; W Lissens; C Ferec; I Liebaers; P Devroey; A C Van Steirteghem Journal: Hum Reprod Date: 1995-08 Impact factor: 6.918