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Literature DB >> 11805338

A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae.

Brian J Akerley¹, Eric J Rubin, Veronica L Novick, Kensey Amaya, Nicholas Judson, John J Mekalanos.

Abstract

A high-density transposon mutagenesis strategy was applied to the Haemophilus influenzae genome to identify genes required for growth or viability. This analysis detected putative essential roles for the products of 259 ORFs of unknown function. Comparisons between complete genomes defined a subset of these proteins in H. influenzae having homologs in Mycobacterium tuberculosis that are absent in Saccharomyces cerevisiae, a distribution pattern that favors their use in development of antimicrobial therapeutics. Three genes within this set are essential for viability in other bacteria. Interfacing the set of essential gene products in H. influenzae with the distribution of homologs in other microorganisms can detect components of unrecognized cellular pathways essential in diverse bacteria. This genome-scale phenotypic analysis identifies potential roles for a large set of genes of unknown function.

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Year: 2002 PMID： 11805338 PMCID： PMC117414 DOI： 10.1073/pnas.012602299

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

30 in total

1. Binding and cross-linking of tmRNA to ribosomal protein S1, on and off the Escherichia coli ribosome.

Authors: I K Wower; C W Zwieb; S A Guven; J Wower
Journal: EMBO J Date: 2000-12-01 Impact factor: 11.598

2. Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA.

Authors: Y Ji; B Zhang; S F Van; P Warren; G Woodnutt; M K Burnham; M Rosenberg
Journal: Science Date: 2001-09-21 Impact factor: 47.728

3. Identification of novel essential Escherichia coli genes conserved among pathogenic bacteria.

Authors: C Freiberg; B Wieland; F Spaltmann; K Ehlert; H Brötz; H Labischinski
Journal: J Mol Microbiol Biotechnol Date: 2001-07

4. Frequency and distribution of DNA uptake signal sequences in the Haemophilus influenzae Rd genome.

Authors: H O Smith; J F Tomb; B A Dougherty; R D Fleischmann; J C Venter
Journal: Science Date: 1995-07-28 Impact factor: 47.728

5. Defined nongrowth media for stage II development of competence in Haemophilus influenzae.

Authors: R M Herriott; E M Meyer; M Vogt
Journal: J Bacteriol Date: 1970-02 Impact factor: 3.490

6. Characterization and virulence analysis of catalase mutants of Haemophilus influenzae.

Authors: W R Bishai; N S Howard; J A Winkelstein; H O Smith
Journal: Infect Immun Date: 1994-11 Impact factor: 3.441

7. SsrA-mediated tagging in Bacillus subtilis.

Authors: T Wiegert; W Schumann
Journal: J Bacteriol Date: 2001-07 Impact factor: 3.490

8. Genetic footprinting with mariner-based transposition in Pseudomonas aeruginosa.

Authors: S M Wong; J J Mekalanos
Journal: Proc Natl Acad Sci U S A Date: 2000-08-29 Impact factor: 11.205

9. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.

Authors: R D Fleischmann; M D Adams; O White; R A Clayton; E F Kirkness; A R Kerlavage; C J Bult; J F Tomb; B A Dougherty; J M Merrick
Journal: Science Date: 1995-07-28 Impact factor: 47.728

10. An eleven-base-pair sequence determines the specificity of DNA uptake in Haemophilus transformation.

Authors: D B Danner; R A Deich; K L Sisco; H O Smith
Journal: Gene Date: 1980-11 Impact factor: 3.688

162 in total

1. Position-based scanning for comparative genomics and identification of genetic islands in Haemophilus influenzae type b.

Authors: Nicholas H Bergman; Brian J Akerley
Journal: Infect Immun Date: 2003-03 Impact factor: 3.441

2. Essential Bacillus subtilis genes.

Authors: K Kobayashi; S D Ehrlich; A Albertini; G Amati; K K Andersen; M Arnaud; K Asai; S Ashikaga; S Aymerich; P Bessieres; F Boland; S C Brignell; S Bron; K Bunai; J Chapuis; L C Christiansen; A Danchin; M Débarbouille; E Dervyn; E Deuerling; K Devine; S K Devine; O Dreesen; J Errington; S Fillinger; S J Foster; Y Fujita; A Galizzi; R Gardan; C Eschevins; T Fukushima; K Haga; C R Harwood; M Hecker; D Hosoya; M F Hullo; H Kakeshita; D Karamata; Y Kasahara; F Kawamura; K Koga; P Koski; R Kuwana; D Imamura; M Ishimaru; S Ishikawa; I Ishio; D Le Coq; A Masson; C Mauël; R Meima; R P Mellado; A Moir; S Moriya; E Nagakawa; H Nanamiya; S Nakai; P Nygaard; M Ogura; T Ohanan; M O'Reilly; M O'Rourke; Z Pragai; H M Pooley; G Rapoport; J P Rawlins; L A Rivas; C Rivolta; A Sadaie; Y Sadaie; M Sarvas; T Sato; H H Saxild; E Scanlan; W Schumann; J F M L Seegers; J Sekiguchi; A Sekowska; S J Séror; M Simon; P Stragier; R Studer; H Takamatsu; T Tanaka; M Takeuchi; H B Thomaides; V Vagner; J M van Dijl; K Watabe; A Wipat; H Yamamoto; M Yamamoto; Y Yamamoto; K Yamane; K Yata; K Yoshida; H Yoshikawa; U Zuber; N Ogasawara
Journal: Proc Natl Acad Sci U S A Date: 2003-04-07 Impact factor: 11.205

A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae.

1. Binding and cross-linking of tmRNA to ribosomal protein S1, on and off the Escherichia coli ribosome.

2. Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA.

3. Identification of novel essential Escherichia coli genes conserved among pathogenic bacteria.

4. Frequency and distribution of DNA uptake signal sequences in the Haemophilus influenzae Rd genome.

5. Defined nongrowth media for stage II development of competence in Haemophilus influenzae.

6. Characterization and virulence analysis of catalase mutants of Haemophilus influenzae.

7. SsrA-mediated tagging in Bacillus subtilis.

8. Genetic footprinting with mariner-based transposition in Pseudomonas aeruginosa.

9. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.

10. An eleven-base-pair sequence determines the specificity of DNA uptake in Haemophilus transformation.

1. Position-based scanning for comparative genomics and identification of genetic islands in Haemophilus influenzae type b.

2. Essential Bacillus subtilis genes.

3. Gene essentiality determines chromosome organisation in bacteria.

4. DEG: a database of essential genes.

5. Ribosylnicotinamide kinase domain of NadR protein: identification and implications in NAD biosynthesis.

Review 6. Determination of the core of a minimal bacterial gene set.

7. UMP kinase from Streptococcus pneumoniae: evidence for co-operative ATP binding and allosteric regulation.

Review 8. Haemophilus influenzae: genetic variability and natural selection to identify virulence factors.

9. NMR assignment of the hypothetical protein HI0004 from Haemophilus influenzae--a putative essential gene product.

10. Ferric uptake regulator and its role in the pathogenesis of nontypeable Haemophilus influenzae.