Literature DB >> 11795805

Resolving isomeric peptide mixtures: a combined HPLC/ion mobility-TOFMS analysis of a 4000-component combinatorial library.

BarnesCatherineA Srebalus1, Amy E Hilderbrand, Stephen J Valentine, David E Clemmer.   

Abstract

A reversed-phase high-performance liquid chromatography (HPLC) separation approach has been combined with ion mobility/time-of-flight (TOF) mass spectrometry in order to characterize a combinatorial peptide library designed to contain 4000 peptides of the general form NH2-Xxx-Xxx-XXX-CO2H, NH2-Ala-Xxx-Xxx-Xxx-CO2H, NH2-Ser-Ala-Xxx-Xxx-Xxx-CO2H and NH2-Leu-Ser-Ala-Xxx-Xxx-Xxx-CO2H (where Xxx represents a randomization over 10 different amino acids: Ala, Arg, Asp, Glu, Gly, Leu, Lys, Phe, Ser, and Val). Addition of the gas-phase mobility separation between the HPLC separation and TOF measurement dimensions makes it possible to resolve many peptide isomers that have identical retention times (and masses).

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Year:  2002        PMID： 11795805     DOI： 10.1021/ac0108562

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  32 in total

1.  Application of ESI-FAIMS-MS to the analysis of tryptic peptides.

Authors:  David A Barnett; Barbara Ells; Roger Guevremont; Randy W Purves
Journal:  J Am Soc Mass Spectrom       Date:  2002-11       Impact factor: 3.109

2.  Tandem mass spectrometric analysis of a mixture of isobars using the survival yield technique.

Authors:  Antony Memboeuf; Laure Jullien; Rémy Lartia; Bernard Brasme; Yves Gimbert
Journal:  J Am Soc Mass Spectrom       Date:  2011-07-19       Impact factor: 3.109

3.  Differentiation of compact and extended conformations of di-ubiquitin conjugates with lysine-specific isopeptide linkages by ion mobility-mass spectrometry.

Authors:  Ji Eun Jung; Nicholas A Pierson; Andreas Marquardt; Martin Scheffner; Michael Przybylski; David E Clemmer
Journal:  J Am Soc Mass Spectrom       Date:  2011-05-24       Impact factor: 3.109

4.  Optimization of the design and operation of FAIMS analyzers.

Authors:  Alexandre A Shvartsburg; Keqi Tang; Richard D Smith
Journal:  J Am Soc Mass Spectrom       Date:  2005-01       Impact factor: 3.109

5.  High-sensitivity ion mobility spectrometry/mass spectrometry using electrodynamic ion funnel interfaces.

Authors:  Keqi Tang; Alexandre A Shvartsburg; Hak-No Lee; David C Prior; Michael A Buschbach; Fumin Li; Aleksey V Tolmachev; Gordon A Anderson; Richard D Smith
Journal:  Anal Chem       Date:  2005-05-15       Impact factor: 6.986

6.  Orthogonal extraction ion mobility spectrometry.

Authors:  Victor V Laiko
Journal:  J Am Soc Mass Spectrom       Date:  2006-02-28       Impact factor: 3.109

7.  Conformation types of ubiquitin [M+8H]8+ Ions from water:methanol solutions: evidence for the N and A States in aqueous solution.

Authors:  Huilin Shi; Nicholas A Pierson; Stephen J Valentine; David E Clemmer
Journal:  J Phys Chem B       Date:  2012-03-02       Impact factor: 2.991

8.  Feasibility of higher-order differential ion mobility separations using new asymmetric waveforms.

Authors:  Alexandre A Shvartsburg; Stefan V Mashkevich; Richard D Smith
Journal:  J Phys Chem A       Date:  2006-03-02       Impact factor: 2.781

9.  High Resolution Separations and Improved Ion Production and Transmission in Metabolomics.

Authors:  Thomas O Metz; Jason S Page; Erin S Baker; Keqi Tang; Jie Ding; Yufeng Shen; Richard D Smith
Journal:  Trends Analyt Chem       Date:  2008-03       Impact factor: 12.296

10.  Rapid and sensitive differentiation of anomers, linkage, and position isomers of disaccharides using High-Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS).

Authors:  Wojciech Gabryelski; Kenneth L Froese
Journal:  J Am Soc Mass Spectrom       Date:  2003-03       Impact factor: 3.109
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