PURPOSE: To estimate dose-volume effects in the rat cervical spinal cord with protons. METHODS AND MATERIALS: Wistar rats were irradiated on the cervical spinal cord with a single fraction of unmodulated protons (150-190 MeV) using the shoot through method, which employs the plateau of the depth-dose profile rather than the Bragg peak. Four different lengths of the spinal cord (2, 4, 8, and 20 mm) were irradiated with variable doses. The endpoint for estimating dose-volume effects was paralysis of fore or hind limbs. RESULTS: The results obtained with a high-precision proton beam showed a marginal increase of ED50 when decreasing the irradiated cord length from 20 mm (ED50 = 20.4 Gy) to 8 mm (ED50 = 24.9 Gy), but a steep increase in ED50 when further decreasing the length to 4 mm (ED50 = 53.7 Gy) and 2 mm (ED50 = 87.8 Gy). These results generally confirm data obtained previously in a limited series with 4-6-MV photons, and for the first time it was possible to construct complete dose-response curves down to lengths of 2 mm. At higher ED50 values and shorter lengths irradiated, the latent period to paralysis decreased from 125 to 60 days. CONCLUSIONS: Irradiation of variable lengths of rat cervical spinal cord with protons showed steeply increasing ED50 values for lengths of less than 8 mm. These results suggest the presence of a critical migration distance of 2-3 mm for cells involved in regeneration processes.
PURPOSE: To estimate dose-volume effects in the rat cervical spinal cord with protons. METHODS AND MATERIALS: Wistar rats were irradiated on the cervical spinal cord with a single fraction of unmodulated protons (150-190 MeV) using the shoot through method, which employs the plateau of the depth-dose profile rather than the Bragg peak. Four different lengths of the spinal cord (2, 4, 8, and 20 mm) were irradiated with variable doses. The endpoint for estimating dose-volume effects was paralysis of fore or hind limbs. RESULTS: The results obtained with a high-precision proton beam showed a marginal increase of ED50 when decreasing the irradiated cord length from 20 mm (ED50 = 20.4 Gy) to 8 mm (ED50 = 24.9 Gy), but a steep increase in ED50 when further decreasing the length to 4 mm (ED50 = 53.7 Gy) and 2 mm (ED50 = 87.8 Gy). These results generally confirm data obtained previously in a limited series with 4-6-MV photons, and for the first time it was possible to construct complete dose-response curves down to lengths of 2 mm. At higher ED50 values and shorter lengths irradiated, the latent period to paralysis decreased from 125 to 60 days. CONCLUSIONS: Irradiation of variable lengths of rat cervical spinal cord with protons showed steeply increasing ED50 values for lengths of less than 8 mm. These results suggest the presence of a critical migration distance of 2-3 mm for cells involved in regeneration processes.
Authors: Daniel Pak; Karen Vineberg; Felix Feng; Randall K Ten Haken; Avraham Eisbruch Journal: Int J Radiat Oncol Biol Phys Date: 2012-01-26 Impact factor: 7.038
Authors: Paul M Medin; Ryan D Foster; Albert J van der Kogel; James W Sayre; William H McBride; Timothy D Solberg Journal: Int J Radiat Oncol Biol Phys Date: 2011-12-22 Impact factor: 7.038
Authors: V E M Mul; J M A de Jong; L H P Murrer; P L A van den Ende; R M A Houben; M Lacko; P Lambin; B G Baumert Journal: Strahlenther Onkol Date: 2011-12-23 Impact factor: 3.621
Authors: Paul M Medin; Ryan D Foster; Albert J van der Kogel; James W Sayre; William H McBride; Timothy D Solberg Journal: Int J Radiat Oncol Biol Phys Date: 2010-10-08 Impact factor: 7.038
Authors: Paul M Medin; Ryan D Foster; Albert J van der Kogel; James W Sayre; William H McBride; Timothy D Solberg Journal: Radiother Oncol Date: 2012-09-14 Impact factor: 6.280
Authors: Paul M Medin; Ryan D Foster; Albert J van der Kogel; Jeffrey Meyer; James W Sayre; Hao Huang; Orhan K Öz Journal: Radiother Oncol Date: 2013-09-20 Impact factor: 6.280