Literature DB >> 11773130

Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa.

Adrienne W Paton1, James C Paton.   

Abstract

We recently described a novel megaplasmid-encoded adhesin produced by certain Shiga toxigenic Escherichia coli (STEC) strains that lack the locus for enterocyte effacement (LEE) pathogenicity island. This adhesin, designated Saa (STEC autoagglutinating adhesin), may be a marker for a subset of LEE-negative STEC strains capable of causing severe gastrointestinal and systemic diseases in humans. In this study, we developed a pentavalent PCR assay for the detection of saa as well as other proven and putative STEC virulence genes (stx1, stx2, eae, and ehxA). The five primer pairs used in the assay do not interfere with each other and generate amplification products of 119, 180, 255, 384, and 534 bp.

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Year:  2002        PMID： 11773130      PMCID： PMC120136          DOI： 10.1128/JCM.40.1.271-274.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  22 in total

1.  Direct detection of Shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction.

Authors:  D Begum; M P Jackson
Journal:  Mol Cell Probes       Date:  1995-08       Impact factor: 2.365

2.  Cloning and characterization of the eae gene of enterohaemorrhagic Escherichia coli O157:H7.

Authors:  J Yu; J B Kaper
Journal:  Mol Microbiol       Date:  1992-02       Impact factor: 3.501

3.  Virulence of enterohemorrhagic Escherichia coli O91:H21 clinical isolates in an orally infected mouse model.

Authors:  S W Lindgren; A R Melton; A D O'Brien
Journal:  Infect Immun       Date:  1993-09       Impact factor: 3.441

4.  Rapid and sensitive method for detection of Shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction.

Authors:  V P Gannon; R K King; J Y Kim; E J Thomas
Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792

5.  Direct detection of Escherichia coli Shiga-like toxin genes in primary fecal cultures by polymerase chain reaction.

Authors:  A W Paton; J C Paton; P N Goldwater; P A Manning
Journal:  J Clin Microbiol       Date:  1993-11       Impact factor: 5.948

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Authors:  M A Karmali
Journal:  Clin Microbiol Rev       Date:  1989-01       Impact factor: 26.132

7.  Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157:H7 infections.

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Journal:  J Infect Dis       Date:  1989-12       Impact factor: 5.226

8.  Detection and characterization of the eae gene of Shiga-like toxin-producing Escherichia coli using polymerase chain reaction.

Authors:  V P Gannon; M Rashed; R K King; E J Thomas
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948

9.  Sequence heterogeneity of the eae gene and detection of verotoxin-producing Escherichia coli using serotype-specific primers.

Authors:  M Louie; J de Azavedo; R Clarke; A Borczyk; H Lior; M Richter; J Brunton
Journal:  Epidemiol Infect       Date:  1994-06       Impact factor: 2.451

10.  Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933.

Authors:  H Schmidt; L Beutin; H Karch
Journal:  Infect Immun       Date:  1995-03       Impact factor: 3.441
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  76 in total

1.  Multiplex PCR assay for the detection of five putative virulence genes encoded in verotoxigenic Escherichia coli plasmids.

Authors:  A V Bustamante; A M Sanso; P M A Lucchesi; A E Parma
Journal:  Curr Microbiol       Date:  2011-01-29       Impact factor: 2.188

2.  Insertions, deletions, and single-nucleotide polymorphisms at rare restriction enzyme sites enhance discriminatory power of polymorphic amplified typing sequences, a novel strain typing system for Escherichia coli O157:H7.

Authors:  Indira T Kudva; Robert W Griffin; Megan Murray; Manohar John; Nicole T Perna; Timothy J Barrett; Stephen B Calderwood
Journal:  J Clin Microbiol       Date:  2004-06       Impact factor: 5.948

3.  Virulence characterization of Shiga-toxigenic Escherichia coli isolates from wholesale produce.

Authors:  Peter C H Feng; Terry Councell; Christine Keys; Steven R Monday
Journal:  Appl Environ Microbiol       Date:  2010-11-05       Impact factor: 4.792

4.  Verotoxins in bovine and meat verotoxin-producing Escherichia coli isolates: type, number of variants, and relationship to cytotoxicity.

Authors:  Alejandra Krüger; Paula M A Lucchesi; Alberto E Parma
Journal:  Appl Environ Microbiol       Date:  2010-10-29       Impact factor: 4.792

5.  Serotypes and virulence profiles of non-O157 Shiga toxin-producing Escherichia coli isolates from bovine farms.

Authors:  Aine Monaghan; Brian Byrne; Séamus Fanning; Torres Sweeney; David McDowell; Declan J Bolton
Journal:  Appl Environ Microbiol       Date:  2011-10-14       Impact factor: 4.792

6.  Multiplex PCR for direct detection of Shiga toxigenic Escherichia coli strains producing the novel subtilase cytotoxin.

Authors:  Adrienne W Paton; James C Paton
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

7.  Prevalences of Shiga toxin subtypes and selected other virulence factors among Shiga-toxigenic Escherichia coli strains isolated from fresh produce.

Authors:  Peter C H Feng; Shanker Reddy
Journal:  Appl Environ Microbiol       Date:  2013-08-30       Impact factor: 4.792

8.  Prevalence and characterization of verotoxin-producing Escherichia coli (VTEC) in cattle from an Ontario abattoir.

Authors:  Musafiri Karama; Roger P Johnson; Robert Holtslander; Scott A McEwen; Carlton L Gyles
Journal:  Can J Vet Res       Date:  2008-07       Impact factor: 1.310

9.  Evidence of pathogenic subgroups among atypical enteropathogenic Escherichia coli strains.

Authors:  Isabel C A Scaletsky; Katia R S Aranda; Tamara B Souza; Neusa P Silva; Mauro B Morais
Journal:  J Clin Microbiol       Date:  2009-09-16       Impact factor: 5.948

10.  Characterisation of verotoxin-producing Escherichia coli strains isolated from human patients in Hungary over a 7-year period.

Authors:  T Mag; N Nógrády; M Herpay; I Tóth; F Rozgonyi
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2009-12-04       Impact factor: 3.267
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