Literature DB >> 15956427

Multiplex PCR for direct detection of Shiga toxigenic Escherichia coli strains producing the novel subtilase cytotoxin.

Adrienne W Paton1, James C Paton.   

Abstract

We have recently described a novel AB5 subtilase cytotoxin produced by certain Shiga toxigenic Escherichia coli (STEC) strains. This potentially lethal toxin may contribute to severe gastrointestinal and systemic disease in humans. In this study we have developed a trivalent PCR assay for the detection of the novel toxin A subunit gene subA, as well as stx1 and stx2. The three primer pairs used in the assay do not interfere with each other and generate amplification products of 556, 180, and 255 bp, respectively. The assay can be used for determining the toxin genotype of STEC isolates, as well as for direct detection of toxin genes in primary fecal culture extracts.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 15956427      PMCID： PMC1151958          DOI： 10.1128/JCM.43.6.2944-2947.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  Characterization of a novel type IV pilus locus encoded on the large plasmid of locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli strains that are virulent for humans.

Authors:  Potjanee Srimanote; Adrienne W Paton; James C Paton
Journal:  Infect Immun       Date:  2002-06       Impact factor: 3.441

Review 2.  Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases.

Authors:  R J Siezen; W M de Vos; J A Leunissen; B W Dijkstra
Journal:  Protein Eng       Date:  1991-10

3.  Characterization of Saa, a novel autoagglutinating adhesin produced by locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli strains that are virulent for humans.

Authors:  A W Paton; P Srimanote; M C Woodrow; J C Paton
Journal:  Infect Immun       Date:  2001-11       Impact factor: 3.441

4.  Haemolytic uraemic syndromes in the British Isles, 1985-8: association with verocytotoxin producing Escherichia coli. Part 2: Microbiological aspects.

Authors:  H Kleanthous; H R Smith; S M Scotland; R J Gross; B Rowe; C M Taylor; D V Milford
Journal:  Arch Dis Child       Date:  1990-07       Impact factor: 3.791

5.  Direct detection of Shiga toxigenic Escherichia coli strains belonging to serogroups O111, O157, and O113 by multiplex PCR.

Authors:  A W Paton; J C Paton
Journal:  J Clin Microbiol       Date:  1999-10       Impact factor: 5.948

6.  Rapid and sensitive method for detection of Shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction.

Authors:  V P Gannon; R K King; J Y Kim; E J Thomas
Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792

Review 7.  Infection by verocytotoxin-producing Escherichia coli.

Authors:  M A Karmali
Journal:  Clin Microbiol Rev       Date:  1989-01       Impact factor: 26.132

8.  Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157:H7 infections.

Authors:  S M Ostroff; P I Tarr; M A Neill; J H Lewis; N Hargrett-Bean; J M Kobayashi
Journal:  J Infect Dis       Date:  1989-12       Impact factor: 5.226

9.  Detection and characterization of the eae gene of Shiga-like toxin-producing Escherichia coli using polymerase chain reaction.

Authors:  V P Gannon; M Rashed; R K King; E J Thomas
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948

10.  A new family of potent AB(5) cytotoxins produced by Shiga toxigenic Escherichia coli.

Authors:  Adrienne W Paton; Potjanee Srimanote; Ursula M Talbot; Hui Wang; James C Paton
Journal:  J Exp Med       Date:  2004-06-28       Impact factor: 14.307
View more
  41 in total

1.  Multiplex PCR assay for the detection of five putative virulence genes encoded in verotoxigenic Escherichia coli plasmids.

Authors:  A V Bustamante; A M Sanso; P M A Lucchesi; A E Parma
Journal:  Curr Microbiol       Date:  2011-01-29       Impact factor: 2.188

2.  Two distinct cytotoxic activities of subtilase cytotoxin produced by shiga-toxigenic Escherichia coli.

Authors:  Naoko Morinaga; Kinnosuke Yahiro; Gen Matsuura; Masaharu Watanabe; Fumio Nomura; Joel Moss; Masatoshi Noda
Journal:  Infect Immun       Date:  2006-11-13       Impact factor: 3.441

3.  The operon encoding SubAB, a novel cytotoxin, is present in shiga toxin-producing Escherichia coli isolates from the United States.

Authors:  A Khaitan; D M Jandhyala; C M Thorpe; J M Ritchie; A W Paton
Journal:  J Clin Microbiol       Date:  2007-02-07       Impact factor: 5.948

4.  Genetic diversity and virulence potential of shiga toxin-producing Escherichia coli O113:H21 strains isolated from clinical, environmental, and food sources.

Authors:  Peter C H Feng; Sabine Delannoy; David W Lacher; Luis Fernando Dos Santos; Lothar Beutin; Patrick Fach; Marta Rivas; Elizabeth L Hartland; Adrienne W Paton; Beatriz E C Guth
Journal:  Appl Environ Microbiol       Date:  2014-05-23       Impact factor: 4.792

5.  Prevalence of the operon encoding subtilase cytotoxin in non-O157 Shiga toxin-producing Escherichia coli isolated from humans in the United States.

Authors:  Jennifer J Wolfson; Dakshina M Jandhyala; Lynn A Gorczyca; Zulekha Qadeer; Shannon D Manning; James Hadler; James T Rudrik; Cheleste M Thorpe
Journal:  J Clin Microbiol       Date:  2009-07-01       Impact factor: 5.948

6.  Activation of the Akt-NF-kappaB pathway by subtilase cytotoxin through the ATF6 branch of the unfolded protein response.

Authors:  Hiroaki Yamazaki; Nobuhiko Hiramatsu; Kunihiro Hayakawa; Yasuhiro Tagawa; Maro Okamura; Ryouji Ogata; Tao Huang; Shotaro Nakajima; Jian Yao; Adrienne W Paton; James C Paton; Masanori Kitamura
Journal:  J Immunol       Date:  2009-06-26       Impact factor: 5.422

7.  Shiga toxin-producing Escherichia coli infection in South India.

Authors:  Priya Rajendran; Deva Prasanna Rajan; Gagandeep Kang; Cheleste M Thorpe
Journal:  J Med Microbiol       Date:  2009-07-09       Impact factor: 2.472

8.  Prevalence and characterization of verotoxin-producing Escherichia coli (VTEC) in cattle from an Ontario abattoir.

Authors:  Musafiri Karama; Roger P Johnson; Robert Holtslander; Scott A McEwen; Carlton L Gyles
Journal:  Can J Vet Res       Date:  2008-07       Impact factor: 1.310

9.  In Vivo leukocyte changes induced by Escherichia coli subtilase cytotoxin.

Authors:  Hui Wang; Adrienne W Paton; Shaun R McColl; James C Paton
Journal:  Infect Immun       Date:  2011-01-31       Impact factor: 3.441

10.  Subtilase cytotoxin-encoding subAB operon found exclusively among Shiga toxin-producing Escherichia coli strains.

Authors:  Kinue Irino; Mônica A Midolli Vieira; Tânia A Tardelli Gomes; Beatriz E Cabilio Guth; Zita V Furtado Naves; Murilo Gomes Oliveira; Luis Fernando dos Santos; Mirian Guirro; Claudio D Timm; Caroline P Pigatto; Sonia M S S Farah; Tânia M I Vaz
Journal:  J Clin Microbiol       Date:  2010-01-20       Impact factor: 5.948
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.