OBJECTIVE: To investigate the influence of heparinized blood vs. native blood on the marginal adaptation of dentin bonded composite resin restorations. METHODS: Cylindrical dentin cavities were prepared in 40 extracted human incisors. After acid-etching four groups (n=10) were contaminated with either fresh capillary blood (FCB), heparinized venous blood (HPB), saline (SAL) or heparinized saline (HPS) and bonded and filled with Scotchbond 1/Z 100. After water storage for 21 days and thermocycling (2000 x, 5-55 degrees C), replicas were produced for evaluation of marginal adaptation by means of quantitative marginal analysis in the SEM. Statistical analysis was performed with the Kruskal-Wallis-test and the Bonferroni correction for multiple comparisons at p<0.05. RESULTS: There were no differences between groups SAL, HPS and HPB. Contamination with fresh capillary blood resulted in significantly higher amounts of marginal openings compared to all other groups. SIGNIFICANCE: Freshly drawn blood has to be used for blood contamination experiments in laboratory studies.
OBJECTIVE: To investigate the influence of heparinized blood vs. native blood on the marginal adaptation of dentin bonded composite resin restorations. METHODS: Cylindrical dentin cavities were prepared in 40 extracted human incisors. After acid-etching four groups (n=10) were contaminated with either fresh capillary blood (FCB), heparinized venous blood (HPB), saline (SAL) or heparinized saline (HPS) and bonded and filled with Scotchbond 1/Z 100. After water storage for 21 days and thermocycling (2000 x, 5-55 degrees C), replicas were produced for evaluation of marginal adaptation by means of quantitative marginal analysis in the SEM. Statistical analysis was performed with the Kruskal-Wallis-test and the Bonferroni correction for multiple comparisons at p<0.05. RESULTS: There were no differences between groups SAL, HPS and HPB. Contamination with fresh capillary blood resulted in significantly higher amounts of marginal openings compared to all other groups. SIGNIFICANCE: Freshly drawn blood has to be used for blood contamination experiments in laboratory studies.
Authors: Ellen Cristina de Carvalho Mendonça; Samuel Nilo Vieira; Fernando Aparecido Kawaguchi; John Powers; Adriana Bona Matos Journal: Eur J Dent Date: 2010-07