Literature DB >> 11752617

Application of the chromatin immunoprecipitation method to identify in vivo protein-DNA associations in fission yeast.

K Takahashi1, S Saitoh, M Yanagida.   

Abstract

The chromatin immunoprecipitation (ChIP) method provides an ideal tool for detecting direct or indirect interactions between proteins of interest and DNAs with known sequences. Here, we introduce the ChIP protocol used in our laboratory to identify in vivo protein-DNA association in the fission yeast Schizosaccharomyces pombe. The cytological and genetic merits of the fission yeast for studying control of the eukaryotic cell cycle and chromosome dynamics are reinforced by application of this ChIP method.

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Year:  2000        PMID： 11752617     DOI： 10.1126/stke.2000.56.pl1

Source DB:  PubMed          Journal:  Sci STKE        ISSN： 1525-8882


  14 in total

1.  A modified chromatin-immunoprecipitation protocol for silkmoth ovarian follicular cells reveals C/EBP and GATA binding modes on an early chorion gene promoter.

Authors:  Argyris Papantonis; Rena Lecanidou
Journal:  Mol Biol Rep       Date:  2008-03-27       Impact factor: 2.316

2.  Homolog of BRCA2-interacting Dss1p and Uap56p link Mlo3p and Rae1p for mRNA export in fission yeast.

Authors:  Anjan G Thakurta; Ganesh Gopal; Jin Ho Yoon; Libor Kozak; Ravi Dhar
Journal:  EMBO J       Date:  2005-06-30       Impact factor: 11.598

3.  Rct1, a nuclear RNA recognition motif-containing cyclophilin, regulates phosphorylation of the RNA polymerase II C-terminal domain.

Authors:  Monika Gullerova; Andrea Barta; Zdravko J Lorkovic
Journal:  Mol Cell Biol       Date:  2007-03-05       Impact factor: 4.272

4.  Fission yeast Dna2 is required for generation of the telomeric single-strand overhang.

Authors:  Kazunori Tomita; Tatsuya Kibe; Ho-Young Kang; Yeon-Soo Seo; Masahiro Uritani; Takashi Ushimaru; Masaru Ueno
Journal:  Mol Cell Biol       Date:  2004-11       Impact factor: 4.272

5.  A novel allele of fission yeast rad11 that causes defects in DNA repair and telomere length regulation.

Authors:  Yuuki Ono; Kazunori Tomita; Akira Matsuura; Takuro Nakagawa; Hisao Masukata; Masahiro Uritani; Takashi Ushimaru; Masaru Ueno
Journal:  Nucleic Acids Res       Date:  2003-12-15       Impact factor: 16.971

6.  Fission yeast Rhp51 is required for the maintenance of telomere structure in the absence of the Ku heterodimer.

Authors:  Tatsuya Kibe; Kazunori Tomita; Akira Matsuura; Daisuke Izawa; Tsutomu Kodaira; Takashi Ushimaru; Masahiro Uritani; Masaru Ueno
Journal:  Nucleic Acids Res       Date:  2003-09-01       Impact factor: 16.971

7.  A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe.

Authors:  Alla Sigova; Nicholas Rhind; Phillip D Zamore
Journal:  Genes Dev       Date:  2004-09-15       Impact factor: 11.361

8.  Fission yeast Arp6 is required for telomere silencing, but functions independently of Swi6.

Authors:  Masaru Ueno; Tadashi Murase; Tatsuya Kibe; Noriyuki Ohashi; Kazunori Tomita; Yota Murakami; Masahiro Uritani; Takashi Ushimaru; Masahiko Harata
Journal:  Nucleic Acids Res       Date:  2004-02-02       Impact factor: 16.971

9.  Fission yeast Taz1 and RPA are synergistically required to prevent rapid telomere loss.

Authors:  Tatsuya Kibe; Yuuki Ono; Koichiro Sato; Masaru Ueno
Journal:  Mol Biol Cell       Date:  2007-04-11       Impact factor: 4.138

10.  Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination.

Authors:  Takamune T Saito; Takahiro Tougan; Takashi Kasama; Daisuke Okuzaki; Hiroshi Nojima
Journal:  Nucleic Acids Res       Date:  2004-06-21       Impact factor: 16.971
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