| Literature DB >> 11747986 |
Takeyoshi Koseki1, Ying Gao, Nobuo Okahashi, Yoshiyuki Murase, Toshiyuki Tsujisawa, Tsuyoshi Sato, Kenji Yamato, Tatsuji Nishihara.
Abstract
Recent studies have revealed that both transforming growth factor-beta (TGF-beta) and activin A play pivotal roles in osteoclastogenesis. In this report, we show that the effect of TGF-beta family members, TGF-beta1 and activin A, but not BMP-2, enhance multinucleated osteoclast-like cell (OCL) formation induced by receptor activator of NF-kappaB ligand (RANKL) in isolated bone marrow macrophages and monocytic cell line, RAW264.7. TGF-beta1 and activin A caused the growth suppression and concomitant expression of tartrate-resistant acid phosphatase (TRAP) and c-Src, without inducing syncytium formation or increasing the survival rate in RAW264.7 cells. Although TGF-beta1 and activin A had no effect on NF-kappaB and JNK activities, these factors enhanced the expression of JunB, a component of the AP-1 transcriptional complex. These results suggest that TGF-beta1 and activin A may function as commitment factors in osteoclastic differentiation, not as a crucial component for terminal differentiation to form multinucleated OCLs nor in OCL survival.Entities:
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Year: 2002 PMID： 11747986 DOI： 10.1016/s0898-6568(01)00221-2
Source DB: PubMed Journal: Cell Signal ISSN： 0898-6568 Impact factor: 4.315