Literature DB >> 11742080

IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins.

C J Schwartz1, J L Giel, T Patschkowski, C Luther, F J Ruzicka, H Beinert, P J Kiley.   

Abstract

IscR (iron-sulfur cluster regulator) is encoded by an ORF located immediately upstream of genes coding for the Escherichia coli Fe-S cluster assembly proteins, IscS, IscU, and IscA. IscR shares amino acid similarity with MarA, a member of the MarA/SoxS/Rob family of transcription factors. In this study, we found that IscR functions as a repressor of the iscRSUA operon, because strains deleted for iscR have increased expression of this operon. In addition, in vitro transcription reactions established a direct role for IscR in repression of the iscR promoter. Analysis of IscR by electron paramagnetic resonance showed that the anaerobically isolated protein contains a [2Fe-2S](1+) cluster. The Fe-S cluster appears to be important for IscR function, because repression of iscR expression is significantly reduced in strains containing null mutations of the Fe-S cluster assembly genes iscS or hscA. The finding that IscR activity is decreased in strain backgrounds in which Fe-S cluster assembly is impaired suggests that this protein may be part of a novel autoregulatory mechanism that senses the Fe-S cluster assembly status of cells.

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Year:  2001        PMID： 11742080      PMCID： PMC64955          DOI： 10.1073/pnas.251550898

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  32 in total

1.  Structural requirements for marbox function in transcriptional activation of mar/sox/rob regulon promoters in Escherichia coli: sequence, orientation and spatial relationship to the core promoter.

Authors:  R G Martin; W K Gillette; S Rhee; J L Rosner
Journal:  Mol Microbiol       Date:  1999-11       Impact factor: 3.501

Review 2.  Oxidative stress.

Authors:  G Storz; J A Imlay
Journal:  Curr Opin Microbiol       Date:  1999-04       Impact factor: 7.934

3.  Assembly of iron-sulfur clusters. Identification of an iscSUA-hscBA-fdx gene cluster from Azotobacter vinelandii.

Authors:  L Zheng; V L Cash; D H Flint; D R Dean
Journal:  J Biol Chem       Date:  1998-05-22       Impact factor: 5.157

4.  One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

Authors:  K A Datsenko; B L Wanner
Journal:  Proc Natl Acad Sci U S A       Date:  2000-06-06       Impact factor: 11.205

5.  Identification of a contact site for different transcription activators in region 4 of the Escherichia coli RNA polymerase sigma70 subunit.

Authors:  M A Lonetto; V Rhodius; K Lamberg; P Kiley; S Busby; C Gross
Journal:  J Mol Biol       Date:  1998-12-18       Impact factor: 5.469

Review 6.  Redox signal transduction via iron-sulfur clusters in the SoxR transcription activator.

Authors:  E Hidalgo; H Ding; B Demple
Journal:  Trends Biochem Sci       Date:  1997-06       Impact factor: 13.807

7.  Iron-sulfur cluster disassembly in the FNR protein of Escherichia coli by O2: [4Fe-4S] to [2Fe-2S] conversion with loss of biological activity.

Authors:  N Khoroshilova; C Popescu; E Münck; H Beinert; P J Kiley
Journal:  Proc Natl Acad Sci U S A       Date:  1997-06-10       Impact factor: 11.205

8.  Modular organization and identification of a mononuclear iron-binding site within the NifU protein.

Authors:  J N Agar; P Yuvaniyama; R F Jack; V L Cash; A D Smith; D R Dean; M K Johnson
Journal:  J Biol Inorg Chem       Date:  2000-04       Impact factor: 3.358

9.  Substitution of leucine 28 with histidine in the Escherichia coli transcription factor FNR results in increased stability of the [4Fe-4S](2+) cluster to oxygen.

Authors:  D M Bates; C V Popescu; N Khoroshilova; K Vogt; H Beinert; E Münck; P J Kiley
Journal:  J Biol Chem       Date:  2000-03-03       Impact factor: 5.157

10.  Escherichia coli contains a protein that is homologous in function and N-terminal sequence to the protein encoded by the nifS gene of Azotobacter vinelandii and that can participate in the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase.

Authors:  D H Flint
Journal:  J Biol Chem       Date:  1996-07-05       Impact factor: 5.157
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  165 in total

1.  Feedback regulation of iron-sulfur cluster biosynthesis.

Authors:  J Frazzon; D R Dean
Journal:  Proc Natl Acad Sci U S A       Date:  2001-12-18       Impact factor: 11.205

2.  Lack of the ApbC or ApbE protein results in a defect in Fe-S cluster metabolism in Salmonella enterica serovar Typhimurium.

Authors:  Elizabeth Skovran; Diana M Downs
Journal:  J Bacteriol       Date:  2003-01       Impact factor: 3.490

3.  Characterization of the Vibrio vulnificus 1-Cys peroxiredoxin Prx3 and regulation of its expression by the Fe-S cluster regulator IscR in response to oxidative stress and iron starvation.

Authors:  Jong Gyu Lim; Ye-Ji Bang; Sang Ho Choi
Journal:  J Biol Chem       Date:  2014-11-14       Impact factor: 5.157

4.  The sufR gene (sll0088 in Synechocystis sp. strain PCC 6803) functions as a repressor of the sufBCDS operon in iron-sulfur cluster biogenesis in cyanobacteria.

Authors:  Tao Wang; Gaozhong Shen; Ramakrishnan Balasubramanian; Lee McIntosh; Donald A Bryant; John H Golbeck
Journal:  J Bacteriol       Date:  2004-02       Impact factor: 3.490

5.  SoxRS-regulated expression and genetic analysis of the yggX gene of Escherichia coli.

Authors:  Pablo J Pomposiello; Anastasia Koutsolioutsou; Daniel Carrasco; Bruce Demple
Journal:  J Bacteriol       Date:  2003-11       Impact factor: 3.490

6.  NMR assignment of HI1723 from Haemophilus influenzae--a sequence homologue from the iron sulfur cluster assembly (IscA) family.

Authors:  Deok Cheon Yeh; Fang Liu; Nicklas Bonander; Edward Eisenstein; John Orban
Journal:  J Biomol NMR       Date:  2004-06       Impact factor: 2.835

Review 7.  Bacterial iron-sulfur regulatory proteins as biological sensor-switches.

Authors:  Jason C Crack; Jeffrey Green; Matthew I Hutchings; Andrew J Thomson; Nick E Le Brun
Journal:  Antioxid Redox Signal       Date:  2012-03-06       Impact factor: 8.401

8.  Structural alterations of the cysteine desulfurase IscS of Salmonella enterica serovar Typhimurium reveal substrate specificity of IscS in tRNA thiolation.

Authors:  Hans K Lundgren; Glenn R Björk
Journal:  J Bacteriol       Date:  2006-04       Impact factor: 3.490

9.  IscR controls iron-dependent biofilm formation in Escherichia coli by regulating type I fimbria expression.

Authors:  Yun Wu; F Wayne Outten
Journal:  J Bacteriol       Date:  2008-12-12       Impact factor: 3.490

10.  Redox control of the DNA damage-inducible protein DinG helicase activity via its iron-sulfur cluster.

Authors:  Binbin Ren; Xuewu Duan; Huangen Ding
Journal:  J Biol Chem       Date:  2008-12-12       Impact factor: 5.157
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