AIMS: To synthesize and evaluate fluorogenic substrates for the detection of microbial nitroreductases. These substrates, all based on 7-nitrocoumarin, may be reduced to form fluorescent aminocoumarins. METHODS AND RESULTS: Thirty pathogenic microbial strains, including both bacteria and yeasts, were examined for nitroreductase activity in a whole-cell assay. All strains readily reduced each of the seven substrates to generate fluorescence, suggesting the widespread presence of nitroreductase activity in pathogenic bacteria. CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: These novel substrates facilitate the direct detection of nitroreductase activity and have potential as sensitive indicators of microbial growth.
AIMS: To synthesize and evaluate fluorogenic substrates for the detection of microbial nitroreductases. These substrates, all based on 7-nitrocoumarin, may be reduced to form fluorescent aminocoumarins. METHODS AND RESULTS: Thirty pathogenic microbial strains, including both bacteria and yeasts, were examined for nitroreductase activity in a whole-cell assay. All strains readily reduced each of the seven substrates to generate fluorescence, suggesting the widespread presence of nitroreductase activity in pathogenic bacteria. CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: These novel substrates facilitate the direct detection of nitroreductase activity and have potential as sensitive indicators of microbial growth.
Authors: Eli M Espinoza; Joachim J Røise; Maomao He; I-Che Li; Alvin K Agatep; Patrick Udenyi; Hesong Han; Nicole Jackson; D Lucas Kerr; Dake Chen; Michael R Stentzel; Emily Ruan; Lee Riley; Niren Murthy Journal: Chem Commun (Camb) Date: 2022-03-03 Impact factor: 6.065
Authors: Anuruddha Rajapakse; Collette Linder; Ryan D Morrison; Ujjal Sarkar; Nathan D Leigh; Charles L Barnes; J Scott Daniels; Kent S Gates Journal: Chem Res Toxicol Date: 2013-04-02 Impact factor: 3.739
Authors: Anzhelika G Vorobyeva; Michael Stanton; Aurélien Godinat; Kjetil B Lund; Grigory G Karateev; Kevin P Francis; Elizabeth Allen; Juri G Gelovani; Emmet McCormack; Mark Tangney; Elena A Dubikovskaya Journal: PLoS One Date: 2015-06-25 Impact factor: 3.240
Authors: Anuruddha Rajapakse; Roman Hillebrand; Sarah M Lewis; Zachary D Parsons; Charles L Barnes; Kent S Gates Journal: Acta Crystallogr Sect E Struct Rep Online Date: 2014-10-11