BACKGROUND: Enhanced stimulus-induced release of pro-inflammatory cytokines by leucocytes may contribute to the pathogenesis of ischaemic stroke. DESIGN: We investigated the lipopolysaccharide-induced release of interleukin-1beta (IL-1beta), IL-6, IL-8, and tumour necrosis factor-alpha (TNF-alpha) in whole blood from 20 patients with a history of ischaemic stroke under the age of 50, 20 patients with a history of cervical artery dissection (CAD) and 21 age- and sex-matched healthy control subjects. RESULTS: Release of IL-8 was higher (P = 0.006) and release of TNF-alpha and IL-6 tended to be higher (P < 0.1) in young stroke patients than in control subjects. No increased release existed in CAD patients. Vascular risk factors or history of infection before stroke did not modify IL-8 production. A common T(250) --> A polymorphism in the IL-8 gene promotor was newly identified but did not correlate with the variability of IL-8 release. The C(260) --> T polymorphism in the gene of the monocytic LPS-receptor CD14--a risk factor for myocardial infarction--was not associated with increased cytokine release. CONCLUSIONS: We conclude that high inducible release of IL-8--and possibly of TNF-alpha and IL-6--may contribute to the odds of ischaemic stroke in young adults.
BACKGROUND: Enhanced stimulus-induced release of pro-inflammatory cytokines by leucocytes may contribute to the pathogenesis of ischaemic stroke. DESIGN: We investigated the lipopolysaccharide-induced release of interleukin-1beta (IL-1beta), IL-6, IL-8, and tumour necrosis factor-alpha (TNF-alpha) in whole blood from 20 patients with a history of ischaemic stroke under the age of 50, 20 patients with a history of cervical artery dissection (CAD) and 21 age- and sex-matched healthy control subjects. RESULTS: Release of IL-8 was higher (P = 0.006) and release of TNF-alpha and IL-6 tended to be higher (P < 0.1) in young strokepatients than in control subjects. No increased release existed in CAD patients. Vascular risk factors or history of infection before stroke did not modify IL-8 production. A common T(250) --> A polymorphism in the IL-8 gene promotor was newly identified but did not correlate with the variability of IL-8 release. The C(260) --> T polymorphism in the gene of the monocytic LPS-receptor CD14--a risk factor for myocardial infarction--was not associated with increased cytokine release. CONCLUSIONS: We conclude that high inducible release of IL-8--and possibly of TNF-alpha and IL-6--may contribute to the odds of ischaemic stroke in young adults.
Authors: M Libra; S S Signorelli; Y Bevelacqua; P M Navolanic; V Bevelacqua; J Polesel; R Talamini; F Stivala; M C Mazzarino; G Malaponte Journal: J Clin Pathol Date: 2006-02 Impact factor: 3.411
Authors: Sofia Markoula; Anthoula Chatzikyriakidou; Sotirios Giannopoulos; Kargiotis Odysseas; Sofia Markou; Konstantinos Vemmos; Ioannis Georgiou; Athanassios P Kyritsis Journal: Stroke Res Treat Date: 2011-05-29
Authors: Hedley C A Emsley; Craig J Smith; Carole M Gavin; Rachel F Georgiou; Andy Vail; Elisa M Barberan; Karen Illingworth; Sylvia Scarth; Vijitha Wickramasinghe; Margaret E Hoadley; Nancy J Rothwell; Pippa J Tyrrell; Stephen J Hopkins Journal: BMC Neurol Date: 2007-02-28 Impact factor: 2.474
Authors: Atif Zafar; Asad Ikram; Dinesh V Jillella; Duraisamy Kempuraj; Mohammad Moshahid Khan; Saif Bushnaq; Harold Adam; Santiago Ortega-Gutierrez; Syed A Quadri; Mudassir Farooqui; Asgar Zaheer; Enrique C Leira Journal: Cureus Date: 2017-10-11