Literature DB >> 11728730

DNA from Aspergillus flavus contains 5-methylcytosine.

H Gowher1, K C Ehrlich, A Jeltsch.   

Abstract

DNA from Aspergillus sp. has been reported not to contain 5-methylcytosine. However, it has been found that Aspergillus nidulans responds to 5-azacytidine, a drug that is a strong inhibitor of DNA methyltransferases. Therefore, we have re-examined the occurrence of 5-methylcytosine in DNA from Aspergillus flavus by using a highly sensitive and specific method for detection of modified bases in genomic DNA comprising high-performance liquid chromatography separation of nucleosides, labeling of the nucleoside with deoxynucleoside kinase and two-dimensional thin-layer chromatography. Our results show that 5-methylcytosine is present in DNA from A. flavus. We estimate the relative amounts of 5-methylcytosine to cytosine to be approximately 1/400.

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Year:  2001        PMID： 11728730     DOI： 10.1111/j.1574-6968.2001.tb10939.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  10 in total

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Review 2.  Epigenome manipulation as a pathway to new natural product scaffolds and their congeners.

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4.  RNA silencing in Aspergillus nidulans is independent of RNA-dependent RNA polymerases.

Authors:  T M Hammond; N P Keller
Journal:  Genetics       Date:  2004-11-15       Impact factor: 4.562

5.  Bisulfite sequencing reveals that Aspergillus flavus holds a hollow in DNA methylation.

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Journal:  PLoS One       Date:  2012-01-20       Impact factor: 3.240

6.  Expression profiling of non-aflatoxigenic Aspergillus parasiticus mutants obtained by 5-azacytosine treatment or serial mycelial transfer.

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7.  Epigenetic Regulation of Verticillium dahliae Virulence: Does DNA Methylation Level Play A Role?

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8.  A cytosine methyltransferase homologue is essential for sexual development in Aspergillus nidulans.

Authors:  Dong W Lee; Michael Freitag; Eric U Selker; Rodolfo Aramayo
Journal:  PLoS One       Date:  2008-06-25       Impact factor: 3.240

Review 9.  Chromatin-Mediated Regulation of Genome Plasticity in Human Fungal Pathogens.

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Journal:  Genes (Basel)       Date:  2019-10-28       Impact factor: 4.141

Review 10.  The Pattern and Function of DNA Methylation in Fungal Plant Pathogens.

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Journal:  Microorganisms       Date:  2020-02-08
  10 in total

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