Literature DB >> 11724872

Automation of fluorescence-based PCR for confirmation of meningococcal disease.

M A Diggle1, G F Edwards, S C Clarke.   

Abstract

A fluorescence-based PCR method was developed, fully automated, and used to confirm infection with Neisseria meningitidis by detection of the meningococcus-specific ctrA gene. The method provided a highly sensitive, high-throughput assay that was reproducible and less labor-intensive than manual methods.

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Year:  2001        PMID： 11724872      PMCID： PMC88576          DOI： 10.1128/JCM.39.12.4518-4519.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  High-throughput single nucleotide polymorphism genotyping by fluorescent 5' exonuclease assay.

Authors:  P A Morin; R Saiz; A Monjazeb
Journal:  Biotechniques       Date:  1999-09       Impact factor: 1.993

Review 2.  The polymerase chain reaction: miracle or mirage? A critical review of its uses and limitations in diagnosis and research.

Authors:  P A Wright; D Wynford-Thomas
Journal:  J Pathol       Date:  1990-10       Impact factor: 7.996

3.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

4.  A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization.

Authors:  D Shalon; S J Smith; P O Brown
Journal:  Genome Res       Date:  1996-07       Impact factor: 9.043

5.  Molecular beacons: probes that fluoresce upon hybridization.

Authors:  S Tyagi; F R Kramer
Journal:  Nat Biotechnol       Date:  1996-03       Impact factor: 54.908

Review 6.  Molecular diagnosis of infectious diseases by nucleic acid hybridization.

Authors:  R P Viscidi; R G Yolken
Journal:  Mol Cell Probes       Date:  1987-03       Impact factor: 2.365

7.  Evaluation of the Applied Biosystems automated Taqman polymerase chain reaction system for the detection of meningococcal DNA.

Authors:  M Guiver; R Borrow; J Marsh; S J Gray; E B Kaczmarski; D Howells; P Boseley; A J Fox
Journal:  FEMS Immunol Med Microbiol       Date:  2000-06

8.  Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.

Authors:  K J Livak; S J Flood; J Marmaro; W Giusti; K Deetz
Journal:  PCR Methods Appl       Date:  1995-06
  8 in total
  4 in total

1.  Evaluation of a fluorescence-based PCR method for identification of serogroup a meningococci.

Authors:  M A Diggle; K Smith; E K Girvan; S C Clarke
Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

Review 2.  The use of hydrolysis and hairpin probes in real-time PCR.

Authors:  Susan M McChlery; Stuart C Clarke
Journal:  Mol Biotechnol       Date:  2003-11       Impact factor: 2.695

3.  Comparison of commercial DNA extraction kits for extraction of bacterial genomic DNA from whole-blood samples.

Authors:  K Smith; M A Diggle; S C Clarke
Journal:  J Clin Microbiol       Date:  2003-06       Impact factor: 5.948

4.  A multi-target real-time PCR assay for rapid identification of meningitis-associated microorganisms.

Authors:  Marco Favaro; Vincenzo Savini; Cartesio Favalli; Carla Fontana
Journal:  Mol Biotechnol       Date:  2013-01       Impact factor: 2.695
  4 in total

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