Literature DB >> 14668539

The use of hydrolysis and hairpin probes in real-time PCR.

Susan M McChlery1, Stuart C Clarke.   

Abstract

In this review we discuss the limitations of traditional PCR methods and introduce real-time PCR, a fluorescence-based detection system, as a method that overcomes these limitations. We describe the technologies involved in real-time PCR including the design of hydrolysis and hairpin probes and the practical applications of such systems.

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Year:  2003        PMID： 14668539     DOI： 10.1385/MB:25:3:267

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  42 in total

1.  Evaluation of CMV viral load using TaqMan CMV quantitative PCR and comparison with CMV antigenemia in heart and lung transplant recipients.

Authors:  M Guiver; A J Fox; K Mutton; N Mogulkoc; J Egan
Journal:  Transplantation       Date:  2001-06-15       Impact factor: 4.939

2.  Multiplex detection of four pathogenic retroviruses using molecular beacons.

Authors:  J A Vet; A R Majithia; S A Marras; S Tyagi; S Dube; B J Poiesz; F R Kramer
Journal:  Proc Natl Acad Sci U S A       Date:  1999-05-25       Impact factor: 11.205

3.  Real time quantitative PCR.

Authors:  C A Heid; J Stevens; K J Livak; P M Williams
Journal:  Genome Res       Date:  1996-10       Impact factor: 9.043

4.  Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis.

Authors:  A S Piatek; S Tyagi; A C Pol; A Telenti; L P Miller; F R Kramer; D Alland
Journal:  Nat Biotechnol       Date:  1998-04       Impact factor: 54.908

5.  Genetic analysis of DNA from single human oocytes: a model for preimplantation diagnosis of cystic fibrosis.

Authors:  C Coutelle; C Williams; A Handyside; K Hardy; R Winston; R Williamson
Journal:  BMJ       Date:  1989-07-01

6.  Relaxation kinetics of dimer formation by self complementary oligonucleotides.

Authors:  M E Craig; D M Crothers; P Doty
Journal:  J Mol Biol       Date:  1971-12-14       Impact factor: 5.469

7.  Quantitative PCR assay to evaluate ampicillin, ofloxacin, and doxycycline for treatment of experimental leptospirosis.

Authors:  Johann Truccolo; Françoise Charavay; Fabrice Merien; Philippe Perolat
Journal:  Antimicrob Agents Chemother       Date:  2002-03       Impact factor: 5.191

8.  Kinetic PCR analysis: real-time monitoring of DNA amplification reactions.

Authors:  R Higuchi; C Fockler; G Dollinger; R Watson
Journal:  Biotechnology (N Y)       Date:  1993-09

9.  Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.

Authors:  P M Holland; R D Abramson; R Watson; D H Gelfand
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-15       Impact factor: 11.205

10.  Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.

Authors:  K J Livak; S J Flood; J Marmaro; W Giusti; K Deetz
Journal:  PCR Methods Appl       Date:  1995-06
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  1 in total

1.  Regulation of granulosa and theca cell transcriptomes during ovarian antral follicle development.

Authors:  Michael K Skinner; Michelle Schmidt; Marina I Savenkova; Ingrid Sadler-Riggleman; Eric E Nilsson
Journal:  Mol Reprod Dev       Date:  2008-09       Impact factor: 2.609
  1 in total

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