Literature DB >> 11722946

Toxicity analysis of N- and C-terminus-deleted vegetative insecticidal protein from Bacillus thuringiensis.

A Selvapandiyan1, N Arora, R Rajagopal, S K Jalali, T Venkatesan, S P Singh, R K Bhatnagar.   

Abstract

A vegetative insecticidal protein (VIP)-encoding gene from a local isolate of Bacillus thuringiensis has been cloned, sequenced, and expressed in Escherichia coli. The expressed protein shows insecticidal activity against several lepidopteran pests but is ineffective against Agrotis ipsilon. Comparison of the amino acid sequence with those of reported VIPs revealed a few differences. Analysis of insecticidal activity with N- and C-terminus deletion mutants suggests a differential mode of action of VIP against different pests.

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Year:  2001        PMID: 11722946      PMCID: PMC93383          DOI: 10.1128/AEM.67.12.5855-5858.2001

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  6 in total

1.  Resistance to Bt toxins.

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Journal:  Science       Date:  2000-01-07       Impact factor: 47.728

Review 2.  Bacillus thuringiensis insecticidal proteins: molecular mode of action.

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3.  toxin-mediated insect resistance in plants.

Authors: 
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5.  Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects.

Authors:  J J Estruch; G W Warren; M A Mullins; G J Nye; J A Craig; M G Koziel
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-28       Impact factor: 11.205

6.  DNA sequencing with chain-terminating inhibitors.

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Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

  6 in total
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2.  Novel Vip3-related protein from Bacillus thuringiensis.

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3.  A deletion mutant ndv200 of the Bacillus thuringiensis vip3BR insecticidal toxin gene is a prospective candidate for the next generation of genetically modified crop plants resistant to lepidopteran insect damage.

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4.  Effects of Site-Mutations Within the 22 kDa No-Core Fragment of the Vip3Aa11 Insecticidal Toxin of Bacillus thuringiensis.

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Review 6.  Bacterial Vegetative Insecticidal Proteins (Vip) from Entomopathogenic Bacteria.

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7.  Conditions for homogeneous preparation of stable monomeric and oligomeric forms of activated Vip3A toxin from Bacillus thuringiensis.

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10.  The mode of action of the Bacillus thuringiensis vegetative insecticidal protein Vip3A differs from that of Cry1Ab delta-endotoxin.

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Journal:  Appl Environ Microbiol       Date:  2003-08       Impact factor: 4.792

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