AIMS: The aim of this study was to determine the growth and survival characteristics for Arcobacter butzleri NCTC 12481. METHODS AND RESULTS: The temperature and pH growth ranges were 15-39 degrees C and pH 6.0-8.0, as determined using impedance microbiology. The maximum specific growth rate was 00.57 h(-1) at 30 degrees C, pH 7.0. Arcobacter butzleri harvested from the exponential phase was more resistant to heat treatment than stationary phase cells (D55 1.1 and 0.4 min, respectively). Fluorescent dye uptake, and the release of UV-absorbing material, increased in heat-treated cells. After 21 d storage at 4 and -20 degrees C, A. butzleri was recovered on blood agar, but not on the isolation media CAT or CCDA. CONCLUSION: Arcobacter butzleri cells from the exponential phase were less heat sensitive than those from the stationary phase. The organism was able to survive cold storage for at least 3 weeks. SIGNIFICANCE AND IMPACT OF THE STUDY: The growth and survival characteristics have been quantified thus providing a greater understanding of this newly emerging pathogen.
AIMS: The aim of this study was to determine the growth and survival characteristics for Arcobacter butzleriNCTC 12481. METHODS AND RESULTS: The temperature and pH growth ranges were 15-39 degrees C and pH 6.0-8.0, as determined using impedance microbiology. The maximum specific growth rate was 00.57 h(-1) at 30 degrees C, pH 7.0. Arcobacter butzleri harvested from the exponential phase was more resistant to heat treatment than stationary phase cells (D55 1.1 and 0.4 min, respectively). Fluorescent dye uptake, and the release of UV-absorbing material, increased in heat-treated cells. After 21 d storage at 4 and -20 degrees C, A. butzleri was recovered on blood agar, but not on the isolation media CAT or CCDA. CONCLUSION:Arcobacter butzleri cells from the exponential phase were less heat sensitive than those from the stationary phase. The organism was able to survive cold storage for at least 3 weeks. SIGNIFICANCE AND IMPACT OF THE STUDY: The growth and survival characteristics have been quantified thus providing a greater understanding of this newly emerging pathogen.
Authors: Christian Scarano; Federica Giacometti; Gerardo Manfreda; Alex Lucchi; Emanuela Pes; Carlo Spanu; Enrico Pietro Luigi De Santis; Andrea Serraino Journal: Appl Environ Microbiol Date: 2014-09-05 Impact factor: 4.792
Authors: Yanán Badilla-Ramírez; Karolina L Fallas-Padilla; Heriberto Fernández-Jaramillo; María Laura Arias-Echandi Journal: Rev Inst Med Trop Sao Paulo Date: 2016-03-22 Impact factor: 1.846
Authors: Graham S Banting; Shannon Braithwaite; Candis Scott; Jinyong Kim; Byeonghwa Jeon; Nicholas Ashbolt; Norma Ruecker; Lisa Tymensen; Jollin Charest; Katarina Pintar; Sylvia Checkley; Norman F Neumann Journal: Appl Environ Microbiol Date: 2016-07-15 Impact factor: 4.792