| Literature DB >> 11714976 |
Shinichi Hatama1,2, Kaori Otake3, Shinya Omoto2, Yasunori Murase2, Atushi Ikemoto2, Masami Mochizuki4, Eiji Takahashi1, Harumi Okuyama2, Yoichi Fujii2.
Abstract
Full-length DNAs of the Coleman and S7801 strains (pSKY3.0, pSKY5.0) of infectious feline foamy viruses (FFVs) were cloned and sequenced. Parental viruses, designated SKY3.0 and SKY5.0, were secreted following transfection of Crandell feline kidney (CRFK) cells. Production of the rescued parental viruses was enhanced in the presence of trichostatin A. Amino acid sequence similarities between FFV and human foamy virus (HFV) are extremely low for the envelope protein and capsid antigen, as predicted from the two clones. However, a chimeric FFV clone was constructed with the HFV Env substituted for the FFV Env. The chimeric virus (HFFV, SKY4.0) was able to infect and replicate in CRFK cells as well as in peripheral blood mononuclear cells of cats in vivo. Consequently, the chimeric HFFV may be useful for the creation of FV vectors for gene transfer strategies.Entities:
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Year: 2001 PMID： 11714976 DOI： 10.1099/0022-1317-82-12-2999
Source DB: PubMed Journal: J Gen Virol ISSN： 0022-1317 Impact factor: 3.891