S Zilin1, L Naifeng, L Bicheng, W Jiping. 1. Division of Endocrinology, ZhongDa Hospital, Southeast University, 210009, Nanjing, China. szl@public1.ptt.js.cn
Abstract
BACKGROUND: Increasing evidence has suggested that advanced glycation end products (AGEs) might play a central role in the pathogenesis of diabetic complications. Serum AGEs concentration may serve as a useful marker for monitoring pathological processes and progression of diabetic complications. METHODS: A flow injection assay (FIA) system was developed using high performance liquid chromatography (HPLC) to detect low molecular mass AGEs (AGE-peptides, AGE-P). Serum from diabetic patients (n=126), normal controls (n=54) and diabetic mice (n=20) and matched controls (n=20) were collected. RESULTS: The coefficient of variance for intra-assay and inter-assay were 1.2% and 6.3%, respectively. The range of recoveries was 94.9-101.9%. The serum AGE-P concentration was significantly increased both in diabetic patients (2.976+/-0.247 vs. 1.385+/-0.131 U/ml, P<0.0001) and mice (6.71+/-0.50 vs. 2.49+/-0.10 U/ml, P<0.0001) than their respective controls. Concentration of AGE-P was positively correlated with serum creatinine (Scr) (r=0.7133, P<0.0001), 24-h urinary protein (24-h UPro) (r=0.8704, P<0.0001) and urinary albumin excretion (UAE) (r=0.5989, P<0.0001). CONCLUSIONS: The present study suggested that FIA might be a reliable method for measuring the serum AGE-P. Furthermore, our results supported the notion that AGE-P might be a valuable marker for predicting the severity of diabetic nephropathy (DN).
BACKGROUND: Increasing evidence has suggested that advanced glycation end products (AGEs) might play a central role in the pathogenesis of diabetic complications. Serum AGEs concentration may serve as a useful marker for monitoring pathological processes and progression of diabetic complications. METHODS: A flow injection assay (FIA) system was developed using high performance liquid chromatography (HPLC) to detect low molecular mass AGEs (AGE-peptides, AGE-P). Serum from diabeticpatients (n=126), normal controls (n=54) and diabeticmice (n=20) and matched controls (n=20) were collected. RESULTS: The coefficient of variance for intra-assay and inter-assay were 1.2% and 6.3%, respectively. The range of recoveries was 94.9-101.9%. The serum AGE-P concentration was significantly increased both in diabeticpatients (2.976+/-0.247 vs. 1.385+/-0.131 U/ml, P<0.0001) and mice (6.71+/-0.50 vs. 2.49+/-0.10 U/ml, P<0.0001) than their respective controls. Concentration of AGE-P was positively correlated with serum creatinine (Scr) (r=0.7133, P<0.0001), 24-h urinary protein (24-h UPro) (r=0.8704, P<0.0001) and urinary albumin excretion (UAE) (r=0.5989, P<0.0001). CONCLUSIONS: The present study suggested that FIA might be a reliable method for measuring the serum AGE-P. Furthermore, our results supported the notion that AGE-P might be a valuable marker for predicting the severity of diabetic nephropathy (DN).
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