A F Fouad1, A W Acosta. 1. Department of Endodontology, University of Connecticut Health Center, Farmington 06030-1715, USA. fouad@nso.uchc.edu
Abstract
AIM: The purpose of this study was to compare periapical lesion progression and the expression of the bone modulating cytokines IL-1alpha, TNF-alpha, IL-4, IL-6 and IL-11 in periapical lesions of normal and C3H/HeJ (LPS hyporesponsive) mice. METHODOLOGY: Pulps of both mandibular first molars from C3H/HeJ and BALB/c (normal) mice were exposed and inoculated with normal mouse oral microorganisms for 2, 4, 6, and 8 weeks. After euthanasia, specimens were prepared for histological examination. A quantitative evaluation of the lesional area and immunohistochemical stain counts was performed. RESULTS: There were no statistically significant differences in progression of periapical lesions for both mouse strains with time (two-factor ANOVA, P > 0.05). The immunohistochemical staining revealed no overall differences between the two strains in levels of expression of the cytokines (P > 0.05). IL-11 expression did not change from control levels in BALB/c mice, but correlated with the expression of IL-6 and IL-4 in C3H/HeJ mice. CONCLUSION: Responsiveness to LPS may not be significant in the pathogenesis of periapical lesions and in cytokine expression within the lesions, when the lesions are induced by non-specific oral flora.
AIM: The purpose of this study was to compare periapical lesion progression and the expression of the bone modulating cytokines IL-1alpha, TNF-alpha, IL-4, IL-6 and IL-11 in periapical lesions of normal and C3H/HeJ (LPS hyporesponsive) mice. METHODOLOGY: Pulps of both mandibular first molars from C3H/HeJ and BALB/c (normal) mice were exposed and inoculated with normal mouse oral microorganisms for 2, 4, 6, and 8 weeks. After euthanasia, specimens were prepared for histological examination. A quantitative evaluation of the lesional area and immunohistochemical stain counts was performed. RESULTS: There were no statistically significant differences in progression of periapical lesions for both mouse strains with time (two-factor ANOVA, P > 0.05). The immunohistochemical staining revealed no overall differences between the two strains in levels of expression of the cytokines (P > 0.05). IL-11 expression did not change from control levels in BALB/c mice, but correlated with the expression of IL-6 and IL-4 in C3H/HeJ mice. CONCLUSION: Responsiveness to LPS may not be significant in the pathogenesis of periapical lesions and in cytokine expression within the lesions, when the lesions are induced by non-specific oral flora.
Authors: Renato Felipe Pereira; Gestter Willian Lattari Tessarin; Fernando Yamamoto Chiba; Maria Sara de Lima Coutinho Mattera; Amanda Gomes Pereira; Thais Verônica Saori Tsosura; Victor Gustavo Balera Brito; Renan Akira Fujii de Oliveira; Edilson Ervolino; Sandra Helena Penha de Oliveira; Luciano Tavares Angelo Cintra; Doris Hissako Matsushita Journal: Saudi Dent J Date: 2021-07-14