Renato Felipe Pereira1, Max Sander de Oliveira da Mota1, Maria Sara de Lima Coutinho Mattera1, Thaís Verônica Saori Tsosura1, Fernando Yamamoto Chiba2, Cléa Adas Saliba Garbin2, Edilson Ervolino1, Luciano Tavares Angelo Cintra3, Maristela Mitiko Okamoto4, Ubiratan Fabres Machado4, Doris Hissako Sumida5. 1. Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Rod. Marechal Rondom, km 527/528, Araçatuba, SP, 16018-805, Brazil. 2. Department of Child and Social Dentistry, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Araçatuba, SP, Brazil. 3. Endodontics, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Araçatuba, SP, Brazil. 4. Department of Physiology and Biophysics, São Paulo Institute of Biomedical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes, 2415, CEP: 05508-900, São Paulo, Brazil. 5. Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Rod. Marechal Rondom, km 527/528, Araçatuba, SP, 16018-805, Brazil. dorishs@foa.unesp.br.
Abstract
OBJECTIVES: Periapical lesion (PL) promotes insulin resistance; however, the mechanisms underlying this alteration are not fully understood. Therefore, in this study, we aimed to evaluate the Akt serine phosphorylation status and GLUT4 expression levels in the gastrocnemius muscle (GM) of rats with PL. MATERIALS AND METHODS: Male Wistar rats (n = 42) were distributed equally into control (CN) and PL groups. The pulpal tissue of the PL group rats was exposed to the oral environment for 30 days. Thereafter, glucose and insulin levels were assessed, followed by homeostasis model assessment of insulin resistance (HOMA-IR). The Akt serine phosphorylation and GLUT4 levels of microsomal (M) and plasma membrane (PM) fractions were evaluated by western blotting and analyzed statistically. RESULTS: Compared to CN group rats, PL group rats had lower insulin sensitivity (as observed by HOMA-IR), lower Akt serine phosphorylation status after insulin stimulus, and lower GLUT4 levels in the PM fraction. However, the M fraction in the PL group did not differ significantly from that of the CN group. CONCLUSIONS: PL decreases insulin sensitivity, Akt phosphorylation, and PM GLUT4 content. CLINICAL RELEVANCE: The present study indicates that preventing endodontic disease can thwart insulin resistance.
OBJECTIVES: Periapical lesion (PL) promotes insulin resistance; however, the mechanisms underlying this alteration are not fully understood. Therefore, in this study, we aimed to evaluate the Aktserine phosphorylation status and GLUT4 expression levels in the gastrocnemius muscle (GM) of rats with PL. MATERIALS AND METHODS: Male Wistar rats (n = 42) were distributed equally into control (CN) and PL groups. The pulpal tissue of the PL group rats was exposed to the oral environment for 30 days. Thereafter, glucose and insulin levels were assessed, followed by homeostasis model assessment of insulin resistance (HOMA-IR). The Aktserine phosphorylation and GLUT4 levels of microsomal (M) and plasma membrane (PM) fractions were evaluated by western blotting and analyzed statistically. RESULTS: Compared to CN group rats, PL group rats had lower insulin sensitivity (as observed by HOMA-IR), lower Aktserine phosphorylation status after insulin stimulus, and lower GLUT4 levels in the PM fraction. However, the M fraction in the PL group did not differ significantly from that of the CN group. CONCLUSIONS: PL decreases insulin sensitivity, Akt phosphorylation, and PM GLUT4 content. CLINICAL RELEVANCE: The present study indicates that preventing endodontic disease can thwart insulin resistance.
Authors: K Watanabe; T Iizuka; A Adeleke; L Pham; A E Shlimon; M Yasin; P Horvath; T G Unterman Journal: J Periodontal Res Date: 2010-09-22 Impact factor: 4.419