L Zhuang1, Z Zhang, Y Guo. 1. Department of Urology, First Hospital, Beijing Medical University, Institute of Urology, Beijing Medical University, Beijing 100034, China.
Abstract
OBJECTIVE: To investigate the expression of growth factor Wnt-5A in urinary tumor cell lines. METHODS: By semi-quantitative RT-PCR (reverse transcriptase polymerase chain reaction), the expression of Wnt-5A in six urinary malignant cell lines, one primary cultured renal fibroblast and one case of normal human renal tissue was detected using Gel Doc 1000 computer controlled molecular analysis system. RESULTS: The expression of Wnt-5A in-urinary tumor cell lines was much higher than that in primary cultured renal fibroblasts (PRF) and normal human renal tissue (NRT). The levels of Wnt-5A mRNA were different between six malignant cell lines. CONCLUSION: The overexpression of growth factor Wnt-5A has a potential effect on the oncogenesis of urinary malignancies.
OBJECTIVE: To investigate the expression of growth factor Wnt-5A in urinary tumor cell lines. METHODS: By semi-quantitative RT-PCR (reverse transcriptase polymerase chain reaction), the expression of Wnt-5A in six urinary malignant cell lines, one primary cultured renal fibroblast and one case of normal human renal tissue was detected using Gel Doc 1000 computer controlled molecular analysis system. RESULTS: The expression of Wnt-5A in-urinary tumor cell lines was much higher than that in primary cultured renal fibroblasts (PRF) and normal human renal tissue (NRT). The levels of Wnt-5A mRNA were different between six malignant cell lines. CONCLUSION: The overexpression of growth factor Wnt-5A has a potential effect on the oncogenesis of urinary malignancies.
Authors: Taylor J Jensen; Ryan J Wozniak; Kylee E Eblin; Sean M Wnek; A Jay Gandolfi; Bernard W Futscher Journal: Toxicol Appl Pharmacol Date: 2008-11-06 Impact factor: 4.219