Literature DB >> 115925

Immunohistological staining of antigens on semithin sections of specimens embedded in plastic (GMA-Quetol 523).

H Takamiya, S R Batsford, J Tokunaga, A Vogt.   

Abstract

Glycol methacrylate-Quetol 523, introduced by Kushida (1977) for combined light and electronmicroscopy studies at low magnification, also permits application of immunofluorescence methods to semithin sections. To recover the antigenicity of proteins fixed with formaldehyde, abrupt dehydration before embedding and subsequent treatment of the semithin sections with protease were essential. Post-staining with suitable histological stains allows exact correlation of antigen localization with tissue structure.

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Year:  1979        PMID： 115925     DOI： 10.1016/0022-1759(79)90102-9

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  7 in total

1.  Intravascular stents: a new technique for tissue processing for histology, immunohistochemistry, and transmission electron microscopy.

Authors:  N Malik; J Gunn; C M Holt; L Shepherd; S E Francis; C M Newman; D C Crossman; D C Cumberland
Journal:  Heart       Date:  1998-11       Impact factor: 5.994

2.  Different fixative-resin combinations and the immunocytochemical demonstration of immunoglobulins (kappa light chains) in semi-thin sections.

Authors:  Y Smart; P R Millard
Journal:  Histochem J       Date:  1985-12

3.  Postembedding immunohistochemical demonstration of antigen in experimental polyarthritis using plastic embedded whole joints.

Authors:  W Hermanns; K Liebig; L C Schulz
Journal:  Histochemistry       Date:  1981-12

4.  Pre-embedding immunohistochemistry as an approach to high resolution antigen localization at the light microscopic level.

Authors:  R M Franklin; M T Martin
Journal:  Histochemistry       Date:  1981

5.  The detection of canine autoantibodies to thyroid antigens by enzyme-linked immunosorbent assay, hemagglutination and indirect immunofluorescence.

Authors:  D M Haines; P M Lording; W J Penhale
Journal:  Can J Comp Med       Date:  1984-07

6.  A simplified plastic embedding and immunohistologic technique for immunophenotypic analysis of human hematopoietic and lymphoid tissues.

Authors:  T T Casey; J B Cousar; R D Collins
Journal:  Am J Pathol       Date:  1988-05       Impact factor: 4.307

7.  The use of Quetol 651 for the post-embedding immunohistochemical demonstration of gamma-aminobutyric acid on semithin sections.

Authors:  J P Denizot; G Arnold; M Geffard; S Libouban
Journal:  Histochem J       Date:  1988-04
  7 in total

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