| Literature DB >> 11591136 |
I Pérez-Arellano1, M Zúñiga, G Pérez-Martínez.
Abstract
A new collection of shuttle cloning vectors has been constructed that can be used in a broad host range, because they carry replication origins which are functional in Escherichia coli (p15A, pWV01, ColE1), Lactococcus lactis, lactobacilli, and Bacillus subtilis (pAMbeta1, pWV01). These plasmids contain the lacZ-T1T2 cassette from pJDC9, which allows the X-gal selection and cloning of DNA fragments that could cause plasmid instability in E. coli. In addition, they have been proved to be structurally and segregationally stable in Lactobacillus casei, in which their copy number has been determined by real-time quantitative PCR. Furthermore, the antibiotic resistance markers (beta-lactamase, chloramphenicol acetyl transferase, and erythromycin transacetylase) and the theta and rolling circle replicating origins have been combined to obtain this set of compatible plasmids (pIA family) that can be cotransformed, both in lactic acid bacteria and in E. coli. Copyright 2001 Academic Press.Entities:
Mesh：
Year: 2001 PMID： 11591136 DOI： 10.1006/plas.2001.1531
Source DB: PubMed Journal: Plasmid ISSN： 0147-619X Impact factor: 3.466