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Literature DB >> 1159083

Subunit structure of factor VIII antigen synthesized by cultured human endothelial cells.

Abstract

Cultured human endothelial cells were labeled with (3H)leucine, and the radioactive Factor VIII antigen present in the postculture medium was isolated by double anitbody immunoprecipitation and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after reduction with dithiothreitol. The Factor VIII antigen synthesized by cultured endothelial cells was found to contain the same single polypeptide subunit (mol wt 225,000) present in plasma Factor VIII antigen. These results suggest that in vivo, the endothelial cell is a major site of synthesis of circulating Factor VIII antigen.

Entities: Chemical Species

Mesh：

Substances：
Antigens
Factor VIII

Year: 1975 PMID： 1159083 PMCID： PMC301918 DOI： 10.1172/JCI108140

Source DB: PubMed Journal: J Clin Invest ISSN： 0021-9738 Impact factor: 14.808

23 in total

1. Dissociation of Factor VIII-related antigen into subunits.

Authors: D E Austen; M Carey; M A Howard
Journal: Nature Date: 1975-01-03 Impact factor: 49.962

2. Degradation of bovine factor 8 by plasmin and trypsin.

Authors: E P Kirby; N Martin; V J Marder
Journal: Blood Date: 1974-05 Impact factor: 22.113

3. Factor VIII of small molecular weight and its aggregation.

Authors: D E Austen
Journal: Br J Haematol Date: 1974-05 Impact factor: 6.998

4. Antihemophilic factor: separation of an active fragment following dissociation by salts or detergents.

Authors: W G Owen; R H Wagner
Journal: Thromb Diath Haemorrh Date: 1972-07-31

5. Molecular forms of antihaemophilic globulin in plasma, cryoprecipitate and after thrombin activation.

Authors: H J Weiss; S Kochwa
Journal: Br J Haematol Date: 1970-01 Impact factor: 6.998

6. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors: K Weber; M Osborn
Journal: J Biol Chem Date: 1969-08-25 Impact factor: 5.157

7. Synthesis of antihemophilic factor antigen by cultured human endothelial cells.

Authors: E A Jaffe; L W Hoyer; R L Nachman
Journal: J Clin Invest Date: 1973-11 Impact factor: 14.808

8. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria.

Authors: E A Jaffe; R L Nachman; C G Becker; C R Minick
Journal: J Clin Invest Date: 1973-11 Impact factor: 14.808

9. Subcellular platelet factor VIII antigen and von Willebrand factor.

Authors: R L Nachman; E A Jaffe
Journal: J Exp Med Date: 1975-05-01 Impact factor: 14.307

10. Molecular analysis of the membrane attack mechanism of complement.

Authors: W P Kolb; J A Haxby; C M Arroyave; H J Müller-Eberhard
Journal: J Exp Med Date: 1972-03-01 Impact factor: 14.307

16 in total

1. Interaction of platelet membrane receptors with von Willebrand factor, ristocetin, and the Fc region of immunoglobulin G.

Authors: A Moore; G D Ross; R L Nachman
Journal: J Clin Invest Date: 1978-11 Impact factor: 14.808

2. Cultured aortic endothelial cells from pigs with von Willebrand disease: in vitro model for studying the molecular defect(s) of the disease.

Authors: F M Booyse; A J Quarfoot; S Bell; D N Fass; J C Lewis; K G Mann; E J Bowie
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

3. Electron-microscopic and immunocytochemical analyses of Weibel-Palade bodies in the human umbilical vein during pregnancy.

Authors: H Kagawa; S Fujimoto
Journal: Cell Tissue Res Date: 1987-09 Impact factor: 5.249

Subunit structure of factor VIII antigen synthesized by cultured human endothelial cells.

1. Dissociation of Factor VIII-related antigen into subunits.

2. Degradation of bovine factor 8 by plasmin and trypsin.

3. Factor VIII of small molecular weight and its aggregation.

4. Antihemophilic factor: separation of an active fragment following dissociation by salts or detergents.

5. Molecular forms of antihaemophilic globulin in plasma, cryoprecipitate and after thrombin activation.

6. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

7. Synthesis of antihemophilic factor antigen by cultured human endothelial cells.

8. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria.

9. Subcellular platelet factor VIII antigen and von Willebrand factor.

10. Molecular analysis of the membrane attack mechanism of complement.

1. Interaction of platelet membrane receptors with von Willebrand factor, ristocetin, and the Fc region of immunoglobulin G.

2. Cultured aortic endothelial cells from pigs with von Willebrand disease: in vitro model for studying the molecular defect(s) of the disease.

3. Electron-microscopic and immunocytochemical analyses of Weibel-Palade bodies in the human umbilical vein during pregnancy.

Review 4. The vascular endothelium-pathobiologic significance.

5. Structural analysis of factor VIII antigen in von Willebrand disease.

6. Characteristics of a clone of endothelial cells derived from a line of normal adult rat lung cells.

7. Bleeding in renal failure: a possible cause.

8. Complex formation of platelet thrombospondin with fibrinogen.

9. Complex formation of platelet thrombospondin with histidine-rich glycoprotein.

10. Rickettsial interactions with human endothelial cells in vitro: adherence and entry.