Y F Yeh1, S L Huang. 1. Institute of Public Health, National Yang-Ming University, 155 Sec. 2, Li-Nong St., Taipei 112, Taiwan.
Abstract
BACKGROUND: Epidemiological studies have suggested that the dietary pattern may be associated with the prevalence of asthma. We previously reported that an increased intake of foods of animal origin was associated with the occurrence of allergic rhinitis and asthma in adolescents. Here we examined the effect of dietary cholesterol in a murine model of allergic pulmonary inflammation. METHODS: Weanling C57BL/6 mice were fed a control diet containing 0.02% cholesterol or a diet supplemented with 1% or 2% cholesterol. Four weeks later the mice were sensitized with intraperitoneal ovalbumin (OVA) followed by OVA or saline inhalation 2 weeks later. OVA aerosol-induced inflammation was significantly enhanced by dietary supplementation of 1% or 2% cholesterol. RESULTS: Among OVA-challenged mice, leukocyte numbers, particularly those of eosinophils, in the bronchoalveolar space increased by 3- to 5-fold with the cholesterol supplement. Among OVA aerosol-challenged mice, the levels of interleukin-5 and cysteinyl leukotrienes in the bronchoalveolar lavage fluid were significantly higher in those fed the 2% cholesterol diet compared with mice on the control diet. CONCLUSIONS: Dietary cholesterol may enhance pulmonary allergic inflammation. Copyright 2001 S. Karger AG, Basel
BACKGROUND: Epidemiological studies have suggested that the dietary pattern may be associated with the prevalence of asthma. We previously reported that an increased intake of foods of animal origin was associated with the occurrence of allergic rhinitis and asthma in adolescents. Here we examined the effect of dietary cholesterol in a murine model of allergic pulmonary inflammation. METHODS: Weanling C57BL/6 mice were fed a control diet containing 0.02% cholesterol or a diet supplemented with 1% or 2% cholesterol. Four weeks later the mice were sensitized with intraperitoneal ovalbumin (OVA) followed by OVA or saline inhalation 2 weeks later. OVA aerosol-induced inflammation was significantly enhanced by dietary supplementation of 1% or 2% cholesterol. RESULTS: Among OVA-challenged mice, leukocyte numbers, particularly those of eosinophils, in the bronchoalveolar space increased by 3- to 5-fold with the cholesterol supplement. Among OVA aerosol-challenged mice, the levels of interleukin-5 and cysteinyl leukotrienes in the bronchoalveolar lavage fluid were significantly higher in those fed the 2% cholesterol diet compared with mice on the control diet. CONCLUSIONS: Dietary cholesterol may enhance pulmonary allergic inflammation. Copyright 2001 S. Karger AG, Basel
Authors: Sherry Weng; Laura Zemany; Kara N Standley; Deborah V Novack; Marie La Regina; Carlos Bernal-Mizrachi; Trey Coleman; Clay F Semenkovich Journal: Proc Natl Acad Sci U S A Date: 2003-05-13 Impact factor: 11.205
Authors: Muriel Smet; Lien Van Hoecke; Ans De Beuckelaer; Seppe Vander Beken; Thomas Naessens; Karl Vergote; Monique Willart; Bart N Lambrecht; Jan-Åke Gustafsson; Knut R Steffensen; Johan Grooten Journal: Immun Inflamm Dis Date: 2016-08-02