Literature DB >> 11574593

Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene.

J Yugueros1, A Temprano, M Sánchez, J M Luengo, G Naharro.   

Abstract

Oligonucleotide primers specific for the Staphylococcus aureus gap gene were previously designed to identify 12 Staphylococcus spp. by PCR. In the present study, AluI digestion of PCR-generated products rendered distinctive restriction fragment length polymorphism patterns that allowed 24 Staphylococcus spp. to be identified with high specificity.

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Year:  2001        PMID: 11574593      PMCID: PMC88409          DOI: 10.1128/JCM.39.10.3693-3695.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  37 in total

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